Publications 2019

Tuberculosis antigen-specific T cell responses during the first 6 months of antiretroviral treatment.
Riou C, Jhilmeet N, Rangaka M, Wilkinson RJ, Wilkinson KA
J Infect Dis. 2019 Aug 17. pii: jiz417. doi: 10.1093/infdis/jiz417. [Epub ahead of print]

Changes in transcript, metabolite and antibody reactivity during the early protective immune response in humans to Mycobacterium tuberculosis infection
January Weiner, Teresa Domaszewska, Simon Donkor, Stefan H E Kaufmann, Philip C Hill, Jayne S Sutherland
Clinical Infectious Diseases, ciz785,
Published: 15 August 2019

Site-Specific DC Surface Signatures Influence CD4+ T Cell Co-stimulation and Lung-Homing
David Pejoski, Marie Ballester, Floriane Auderset, Maria Vono, Dennis Christensen, Peter Andersen, Paul-Henri Lambert, and Claire-Anne Siegrist.
Front. Immunol., 18 July 2019 |

Comparative Metabolomics between Mycobacterium tuberculosis and the MTBVAC Vaccine Candidate.
Díaz C, Pérez De Palacio J, Valero-Guillén PL, Mena García P, Pérez I, Vicente F, Martín C, Genilloud O, Sánchez Pozo A, Gonzalo-Asensio J.
ACS Infect Dis. 2019 Aug 9;5(8):1317-1326. doi: 10.1021/acsinfecdis.9b00008. Epub 2019 May 30.

Humanized Mouse Model Mimicking Pathology of Human Tuberculosis for in vivo Evaluation of Drug Regimens
Frida Arrey1, Delia Löwe, Stefanie Kuhlmann, Peggy Kaiser, Pedro Moura-Alves, Gopinath Krishnamoorthy, Laura Lozza, Jeroen Maertzdorf, Tatsiana Skrahina1, Alena Skrahina, Martin Gengenbacher, Geraldine Nouailles and Stefan H. E. Kaufmann
Front. Immunol., 31 January 2019

Prevention of tuberculosis infection and disease by local BCG in repeatedly exposed rhesus macaques
Karin Dijkman, Claudia C. Sombroek, Richard A. W. Vervenne, Sam O. Hofman, Charelle Boot, Edmond J. Remarque, Clemens H. M. Kocken, Tom H. M. Ottenhoff, Ivanela Kondova, Mohammed A. Khayum, Krista G. Haanstra, Michel P. M. Vierboom & Frank A. W. Verreck
Nature Medicine volume 25, pages255–262 (2019) Published: 21 January 2019

An NAD+ Phosphorylase Toxin Triggers Mycobacterium tuberculosis Cell Death
Diana Mendes Freire, Claude Gutierrez, Acely Garza-Garcia Anna D.Grabowska, Ambre J.Sala, Kanchiyaphat Ariyachaokun, Terezie Panikova, Katherine S.H.Beckham, André Colom, Vivian Pogenberg, Michele Cianci, Anne Tuukkanen, Yves-Marie Boudehen, Antonio Peixoto, Laure Botella Dmitri I.Svergun, Dirk Schnappinger, Thomas R.Schneider, Olivier Neyrolles
Molecular Cell Volume 73, Issue 6, 21 March 2019, Pages 1282-1291.e8

Publications 2018

Atypical Human Effector/Memory CD4+ T Cells With a Naive-Like Phenotype
Nadia Caccamo, Simone A. Joosten, Tom H. M. Ottenhoff and Francesco Dieli
Front. Immunol., 03 December 2018 |

HBHA-Induced Polycytotoxic CD4+ T Lymphocytes Are Associated with the Control of Mycobacterium tuberculosis Infection in Humans.
Aerts L, Selis E, Corbière V, Smits K, Van Praet A, Dauby N, Petit E,Singh M, Locht C, Dirix V, Mascart F
J Immunol. 2019 Jan 15;202(2):421-427. doi: 10.4049/jimmunol.1800840. Epub 2018 Dec 17.

Complement Component C1q as Serum Biomarker to Detect Active Tuberculosis
Rosalie Lubbers, Jayne S. Sutherland, Delia Goletti, Roelof A. de Paus, Coline H. M. van Moorsel, Marcel Veltkamp, Stefan M. T. Vestjens, Willem J. W. Bos, Linda Petrone, Franca Del Nonno, Ingeborg M. Bajema, Karin Dijkman, Frank A. W. Verreck, Gerhard Walzl, Kyra A. Gelderman, Geert H. Groeneveld, Annemieke Geluk, Tom H. M. Ottenhoff, Simone A. Joosten and Leendert A. Trouw
Front. Immunol., 23 October 2018 |

Lipid conjugation of TLR7 agonist Resiquimod ensures co-delivery with the liposomal Cationic Adjuvant Formulation 01 (CAF01) but does not enhance immunopotentiation compared to non-conjugated Resiquimod+CAF01.
Wilkinson A, Lattmann E, Roces CB, Pedersen GK, Christensen D, Perrie Y.
J Control Release. 2018 Dec 10;291:1-10. doi: 10.1016/j.jconrel.2018.10.002. Epub 2018 Oct 3.

Plasma Biomarkers to Detect Prevalent or Predict Progressive Tuberculosis Associated With Human Immunodeficiency Virus–1
Maia Lesosky, Molebogeng Rangaka, Cara Pienaar, Anna K Coussens, Rene Goliath, Shaheed Mathee, Judith Mwansa-Kambafwile, Gary Maartens, Robert J Wilkinson, Katalin Andrea Wilkinson
Clinical Infectious Diseases, Volume 69, Issue 2, 15 July 2019, Pages 295–305,

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo.
Holm LL, Vukmanovic-Stejic M, Blauenfeldt T, Benfield T, Andersen P, Akbar AN, Ruhwald M.
J Vis Exp. 2018 Aug 11;(138). doi: 10.3791/57554.

First description of agonist and antagonist IP-10 in urine of patients with active TB.
Petrone L, Bondet V, Vanini V, Cuzzi G, Palmieri F, Palucci I, Delogu G, Ciccosanti F, Fimia GM, Blauenfeldt T, Ruhwald M, Duffy D, Goletti D.
Int J Infect Dis. 2018 Sep 7;78:15-21. doi: 10.1016/j.ijid.2018.09.001. [Epub ahead of print]

Escaping the Plato’s cave of latent tuberculosis testing: a path for developers of predictive tests for risk of tuberculosis.
Morten Ruhwald, Roland Diel
European Respiratory Journal 2018 52: 1801616; DOI: 10.1183/13993003.01616-2018

Interplay of DDP4 and IP-10 as a Potential Mechanism for Cell Recruitment to Tuberculosis Lesions.
Blauenfeldt T, Petrone L, Del Nonno F, Baiocchini A, Falasca L, Chiacchio T, Bondet V, Vanini V, Palmieri F, Galluccio G, Casrouge A, Eugen-Olsen J, Albert ML, Goletti D, Duffy D, Ruhwald M.
Front Immunol. 2018 Jul 5;9:1456. doi: 10.3389/fimmu.2018.01456. eCollection 2018.

Author information
Minimal Information About an Immuno-Peptidomics Experiment (MIAIPE).
Lill JR, van Veelen PA, Tenzer S, Admon A, Caron E, Elias JE, Heck AJR, Marcilla M, Marino F, Müller M, Peters B, Purcell A, Sette A, Sturm T, Ternette N, Vizcaíno JA, Bassani-Sternberg M.
Proteomics. 2018 Jun;18(12):e1800110. doi: 10.1002/pmic.201800110.

Unexpected genomic and phenotypic diversity of Mycobacterium africanum Lineage 5 affects drug resistance, protein secretion and immunogenicity
Louis S Ates Anzaan Dippenaar Fadel Sayes Alexandre Pawlik Christiane Bouchier Laurence Ma Robin M Warren Wladimir Sougakoff Laleh Majlessi Jeroen W J van Heijst Florence Brossier Roland Brosch
Genome Biology and Evolution, evy145,

RD5-mediated lack of PE_PGRS and PPE-MPTR export in BCG vaccine strains results in strong reduction of antigenic repertoire but little impact on protection
Louis S. Ates , Fadel Sayes, Wafa Frigui, Roy Ummels, Merel P. M. Damen, Daria Bottai, Marcel A. Behr, Jeroen W. J. van Heijst, Wilbert Bitter, Laleh Majlessi, Roland Brosch
PLos Pathogens, Published: June 18, 2018

Clinical isolates of the modern Mycobacterium tuberculosis lineage 4 evade host defense in human macrophages through eluding IL-1β-induced autophagy
Alessandra Romagnoli, Elisa Petruccioli, Ivana Palucci, Serena Camassa, Elisabetta Carata, Linda Petrone, Stefania Mariano, Michela Sali, Luciana Dini, Enrico Girardi, Giovanni Delogu, Delia Goletti & Gian Maria Fimia
Cell Death & Disease volume 9, Article number: 624 (2018)

Human CD4 T-Cells With a Naive Phenotype Produce Multiple Cytokines During Mycobacterium Tuberculosis Infection and Correlate With Active Disease
Valentina Orlando, Marco P. La Manna1, Delia Goletti, Fabrizio Palmieri, Elena Lo Presti, Simone A. Joosten, Carmela La Mendola, Simona Buccheri, Tom H. M. Ottenhoff, Francesco Dieli and Nadia Caccamo.
Front. Immunol., 23 May 2018 |

Mycobacterium tuberculosis Invasion of the Human Lung: First Contact.
Maertzdorf J, Tönnies M, Lozza L, Schommer-Leitner S, Mollenkopf H, Bauer TT, Kaufmann SHE.
Front Immunol. 2018 Jun 12;9:1346. doi: 10.3389/fimmu.2018.01346. eCollection 2018.

Rv0613c/MSMEG_1285 Interacts with HBHA and Mediates Its Proper Cell-Surface Exposure in Mycobacteria.
Veyron-Churlet R, Dupres V, Saliou JM, Lafont F, Raze D, Locht C.
Int J Mol Sci. 2018 Jun 5;19(6). pii: E1673. doi: 10.3390/ijms19061673.

A multi-antigenic MVA vaccine increases efficacy of combination chemotherapy against Mycobacterium tuberculosis
Stéphane Leung-Theung-Long , Charles-Antoine Coupet, Marie Gouanvic, Doris Schmitt, Aurélie Ray, Chantal Hoffmann, Huguette Schultz, Sandeep Tyagi, Heena Soni, Paul J. Converse, Lilibeth Arias, Patricia Kleinpeter, Benoît Sansas, Khisimuzi Mdluli, Cristina Vilaplana, Pere-Joan Cardona, Eric Nuermberger, Jean-Baptiste Marchand, Nathalie Silvestre, Geneviève Inchauspé
PLos One, Published: May 2, 2018

Multiplexed Quantitation of Intraphagocyte Mycobacterium tuberculosis Secreted Protein Effectors.
Sayes F, Blanc C, Ates LS, Deboosere N, Orgeur M, Le Chevalier F, Gröschel MI, Frigui W, Song OR, Lo-Man R, Brossier F, Sougakoff W, Bottai D, Brodin P, Charneau P, Brosch R, Majlessi L.
Cell Rep. 2018 Apr 24;23(4):1072-1084. doi: 10.1016/j.celrep.2018.03.125.

Contribution of APCs to mucosal-associated invariant T cell activation in infectious disease and cancer.
Shey MS, Balfour A, Wilkinson KA, Meintjes G.
Innate Immun. 2018 May;24(4):192-202. doi: 10.1177/1753425918768695. Epub 2018 Apr 9.

New insights into the transposition mechanisms of IS6110 and its dynamic distribution between Mycobacterium tuberculosis Complex lineages
Jesús Gonzalo-Asensio , Irene Pérez, Nacho Aguiló, Santiago Uranga, Ana Picó, Carlos Lampreave, Alberto Cebollada, Isabel Otal, Sofía Samper, Carlos Martín
PLos Genetics, Published: April 12, 2018

Four-gene Pan-African Blood Signature Predicts Progression to Tuberculosis.
Suliman S, Thompson E, Sutherland J, Weiner Rd J, Ota MOC, Shankar S, Penn-Nicholson A, Thiel B, Erasmus M, Maertzdorf J, Duffy FJ, Hill PC, Hughes EJ, Stanley K, Downing K, Fisher ML, Valvo J, Parida SK, van der Spuy G, Tromp G, Adetifa IMO, Donkor S, Howe R, Mayanja-Kizza H, Boom WH, Dockrell H, Ottenhoff THM, Hatherill M, Aderem A, Hanekom WA, Scriba TJ, Kaufmann SH, Zak DE, Walzl G; and the GC6-74 and ACS cohort study groups.
Am J Respir Crit Care Med. 2018 Apr 6. doi: 10.1164/rccm.201711-2340OC.

Comparative ‘omics analyses differentiate Mycobacterium tuberculosis and Mycobacterium bovis and reveal distinct macrophage responses to infection with the human and bovine tubercle bacilli.
Malone KM, Rue-Albrecht K, Magee DA, Conlon K, Schubert OT, Nalpas NC, Browne JA, Smyth A, Gormley E, Aebersold R, MacHugh DE, Gordon SV.
Microb Genom. 2018 Mar 20. doi: 10.1099/mgen.0.000163

Progress and challenges in TB vaccine development
Gerald Voss, Danilo Casimiro, Olivier Neyrolles, Ann Williams, Stefan H.E. Kaufmann, Helen McShane, Mark Hatherill, and Helen A Fletcher
F1000 Res. 2018; 7: 199.Published online 2018 Feb 16. doi: 10.12688/f1000research.13588.1

Identification of plasma biomarkers for discrimination between tuberculosis infection/disease and pulmonary non tuberculosis disease
Marco Pio La Manna , Valentina Orlando , Paolo Li Donni, Guido Sireci, Paola Di Carlo, Antonio Cascio, Francesco Dieli , Nadia Caccamo
PloSOne, Published: March 15, 2018

High monocyte to lymphocyte ratio is associated with impaired protection after subcutaneous administration of BCG in a mouse model of tuberculosis [version 1; referees: 1 approved, 1 approved with reservations]
Andrea Zelmer, Lisa Stockdale1, Satria A. Prabowo, Felipe Cia1, Natasha Spink1, Matthew Gibb, Ayad Eddaoudi3, Helen A. Fletcher

T-cell biomarkers for diagnosis of tuberculosis: candidate evaluation by a simple whole blood assay for clinical translation.
Musvosvi M, Duffy D, Filander E, Africa H, Mabwe S, Jaxa L, Bilek N, Llibre A, Rouilly V, Hatherill M, Albert M, Scriba TJ, Nemes E
Eur Respir J. 2018 Mar 22;51(3). pii: 1800153. doi: 10.1183/13993003.00153-2018. Print 2018 Mar.

Data-driven model for the assessment of Mycobacterium tuberculosis transmission in evolving demographic structures
Sergio Arreguia, Maria Jose Iglesias, Sofıa Samperd, Dessislava Marinovac, Carlos Martin, Joaquın Sanz and Yamir Moreno
PNAS April 3, 2018. 115 (14) E3238-E3245; published ahead of print March 21, 2018.

Blood tolerogenic monocytes and low proportions of dendritic cell subpopulations are hallmarks of human tuberculosis.
Dirix V, Corbière V, Wyndham-Thomas C, Selis E, Allard S, Hites M, Aerts L, Giese T, Mascart F.
J Leukoc Biol. 2018 May;103(5):945-954. doi: 10.1002/JLB.4A1117-448R. Epub 2018 Feb 28.

Impaired IFN-α-mediated signal in dendritic cells differentiates active from latent tuberculosis
Stefania Parlato, Teresa Chiacchio, Debora Salerno, Linda Petrone, Luciano Castiello, Giulia Romagnoli, Irene Canini, Delia Goletti , Lucia Gabriele
Plus One,  Published: January 10, 2018

Functional, Antigen-Specific Stem Cell Memory (TSCM) CD4+ T Cells Are Induced by Human Mycobacterium tuberculosis Infection.
Mpande CAM, Dintwe OB, Musvosvi M, Mabwe S, Bilek N, Hatherill M, Nemes E, Scriba TJ; SATVI Clinical Immunology Team.
Front Immunol. 2018 Mar 1;9:324. doi: 10.3389/fimmu.2018.00324. eCollection 2018.

The Multirole of Liposomes in Therapy and Prevention of Infectious Diseases
Roberto Nisini1, Noemi Poerio, Sabrina Mariotti, Federica De Santis and Maurizio Fraziano
Front. Immunol., 05 February 2018 |

The Immune Response to Mycobacterium tuberculosis in HIV-1-Coinfected Persons.
Esmail H, Riou C, Bruyn ED, Lai RP, Harley YXR, Meintjes G, Wilkinson KA, Wilkinson RJ
Annu Rev Immunol. 2018 Apr 26;36:603-638. doi: 10.1146/annurev-immunol-042617-053420. Epub 2018 Feb 28.

Mycobacterial growth inhibition is associated with trained innate immunity
Simone A. Joosten, Krista E. van Meijgaarden, Sandra M. Arend, Corine Prins, Fredrik Oftung, Gro Ellen Korsvold, Sandra V. Kik, Rob J.W. Arts, Reinout van Crevel, Mihai G. Netea, and Tom H.M. Ottenhoff
J Clin Invest. 2018;128(5):1837–1851. Published in Volume 128, Issue 5 (May 1, 2018)

Copyright © 2018, American Society for Clinical Investigation
Combined chemical genetics and data-driven bioinformatics approach identifies receptor tyrosine kinase inhibitors as host-directed antimicrobials.
Korbee CJ, Heemskerk MT, Kocev D, van Strijen E, Rabiee O, Franken KLMC, Wilson L, Savage NDL, Džeroski S, Haks MC, Ottenhoff THM.
Nat Commun. 2018 Jan 24;9(1):358. doi: 10.1038/s41467-017-02777-6.

Experimental infection of cattle with Mycobacterium tuberculosis isolates shows the attenuation of the human tubercle bacillus for cattle
Bernardo Villarreal-Ramos, Stefan Berg, Adam Whelan, Sebastien Holbert, Florence Carreras, Francisco J. Salguero, Bhagwati L. Khatri, Kerri Malone, Kevin Rue-Albrecht, Ronan Shaughnessy, Alicia Smyth, Gobena Ameni, Abraham Aseffa, Pierre Sarradin, Nathalie Winter, Martin Vordermeier & Stephen V. Gordon
Scientific Reports volume 8, Article number: 894 (2018)

Publications 2017

TBVAC2020: Advancing Tuberculosis Vaccines from Discovery to Clinical Development
Stefan H. E. Kaufmann, Hazel M. Dockrell, Nick Drager, Mei Mei Ho, Helen McShane, Olivier Neyrolles, Tom H. M. Ottenhoff, Brij Patel, Danielle Roordink, François Spertini, Steffen Stenger, Jelle Thole, Frank A. W. Verreck, Ann Williams and TBVAC2020 Consortium
Front. Immunol., ; 04 October 2017

The E3 ubiquitin ligase NEDD4 enhances killing of membrane-perturbing intracellular bacteria by promoting autophagy
Gang Pei, Hellen Buijze, Haipen Liu, Pedro Moura-Alves, Volker Brinkmann, Stefan Kaufmann
Research Paper-Basic Science; Volume 13, 2017 – Issue 12

Urine lipoarabinomannan glycan in HIV-negative patients with pulmonary tuberculosis correlates with disease severity
Luisa Paris, Ruben Magni, Fatima Zaidi, Robyn Araujo, Neal Saini, Michael Harpole, Jorge Coronel, Daniela E. Kirwan, Hannah Steinberg, Robert H. Gilman, Emanuel F. Petricoin III, Roberto Nisini, Alessandra Luchini,* and Lance Liotta
Science Translational Medicine 13 Dec 2017: Vol. 9, Issue 420, eaal2807 DOI: 10.1126/scitranslmed.aal2807

T Cells Primed by Live Mycobacteria Versus a Tuberculosis Subunit
Thomas Lindenstrøm, Albanus Moguche, Mie Damborg, Else Marie Agger, Kevin Urdah, Peter Andersen
EBioMedicine, Volume 27, January 2018, Pages 27-39

MTBVAC: Attenuating the Human Pathogen of Tuberculosis (TB) Toward a Promising Vaccine against the TB Epidemic
Jesus Gonzalo-Asensio, Dessislava Marinova, Carlos Martin, and Nacho Aguilo
Front. Immunol., 15 December 2017 |

Application of Pharmacokinetics Modelling to Predict Human Exposure of a Cationic Liposomal Subunit Antigen Vaccine System.
Badhan RKS, Khadke S, Perrie Y.
Pharmaceutics. 2017 Dec 7;9(4). pii: E57. doi: 10.3390/pharmaceutics9040057.

Mutations in ppe38 block PE_PGRS secretion and increase virulence of Mycobacterium tuberculosis
Luuis S. Ates, Anzaan Dippenaar, Roy Ummels, Sander R. Piersma, Aniek D. van der Woude, Kim van der Kuij, Fabien Le Chevalier, Dulce Mata-Espinosa, Jorge Barrios-Payán, Brenda Marquina-Castillo, Carolina Guapillo, Connie R. Jiménez, Arnab Pain, Edith N. G. Houben, Robin M. Warren, Roland Brosch, Rogelio Hernández-Pando & Wilbert Bitter
Nature Microbiology volume 3, pages181–188 (2018)

The Biology and Epidemiology of Mycobacterium canettii.
Supply P, Brosch R.
Adv Exp Med Biol. 2017;1019:27-41. doi: 10.1007/978-3-319-64371-7_2.

The Macrophage: A Disputed Fortress in the Battle against Mycobacterium tuberculosis.
Queval CJ, Brosch R, Simeone R.
Front Microbiol. 2017 Nov 23;8:2284. doi: 10.3389/fmicb.2017.02284. eCollection 2017.

B Cells Producing Type I IFN Modulate Macrophage Polarization in Tuberculosis.
Bénard A, Sakwa I, Schierloh P, Colom A, Mercier I, Tailleux L, Jouneau L, Boudinot P, Al-Saati T, Lang R, Rehwinkel J, Loxton AG, Kaufmann SHE, Anton-Leberre V, O’Garra A, Sasiain MDC, Gicquel , Fillatreau S, Neyrolles O, Hudrisier D.
Am J Respir Crit Care Med. 2018 Mar 15;197(6):801-813. doi: 10.1164/rccm.201707-1475OC.

A method permissive to fixation and permeabilization for the multiparametric analysis of apoptotic and necrotic cell phenotype by flow cytometry.
Sabrina Mariotti, Manuela Pardini, Raffaela Teloni, Maria C. Gagliardi, Maurizio Fraziano, Roberto Nisini
Cytometry A. 2017 Nov;91(11):1115-1124. doi: 10.1002/cyto.a.23268. Epub 2017 Oct 26.

Host-directed therapies for bacterial and viral infections
Stefan H. E. Kaufmann, Anca Dorhoi, Richard S. Hotchkiss and Ralf Bartenschlager
Nature reviews drug discovery Vol. 17 January 2018, 35

Immune characterization of the HBHA-specific response in Mycobacterium tuberculosis-infected patients with or without HIV infection
Teresa Chiacchio, Giovanni Delogu, Valentina Vanini, Gilda Cuzzi, Flavio De Maio,Carmela Pinnetti, Alessandro Sampaolesi, Andrea Antinori, Delia Goletti
Plos One; August 24, 2017

Mycobacterium tuberculosis Controls Phagosomal Acidification by Targeting CISH-Mediated Signaling
Christophe J. Queval Ok-Ryul Song, Jean-Philippe Carralot, Jean-Michel Saliou, Antonino Bongiovanni, Gaspard Deloison, Nathalie Deboosère, Samuel Jouny, Raffaella Iantomasi, Vincent Delorme, Anne-Sophie Debrie, Park Sei-Jin, Joana Costa Gouveia, Stanislas Tomavo, Roland Brosch, Akihiko Yoshimura, Edouard Yeramian and Priscille Brodin
Cell report Volume 20, Issue 13, 26 September 2017, Pages 3188-3198

Age-Stratified T Cell Responses in Children Infected with Mycobacterium tuberculosis
A. Dreesman, V. Corbière, V. Dirix, K. Smits, S. Debulpaep, I. De Schutter, M.Libin, M. Singh, A. Malfroot, C. Locht, F. Mascart
Front. Immunol., 05 September 2017 |

The SysteMHC Atlas project.
Shao W, Pedrioli PGA, Wolski W, Scurtescu C, Schmid E, Vizcaíno JA, Courcelles M, Schuster H,Kowalewski D, Marino F, Arlehamn CSL, Vaughan K, Peters B, Sette A, Ottenhoff THM, Meijgaarden KE, Nieuwenhuizen N, Kaufmann SHE, Schlapbach R, Castle JC, Nesvizhskii AI, Nielsen M, Deutsch EW, Campbell DS, Moritz RL, Zubarev RA, Ytterberg AJ, Purcell AW, Marcilla M, Paradela A, Wang Q, Costello CE, Ternette N, van Veelen PA, van Els CACM, Heck AJR, de Souza GA, Sollid LM, Admon A, Stevanovic S, Rammensee HG, Thibault P, Perreault C, Bassani-Sternberg M, Aebersold R, Caron E.
Nucleic Acids Res. 2018 Jan 4;46(D1):D1237-D1247. doi: 10.1093/nar/gkx664.

A Case for a Human Immuno-Peptidome Project Consortium.
Caron E, Aebersold R, Banaei-Esfahani A, Chong C, Bassani-Sternberg M.
Immunity. 2017 Aug 15;47(2):203-208. doi: 10.1016/j.immuni.2017.07.010.

BCG — old workhorse, new skills
M Gengenbacher, NE Nieuwenhuizen and SHE Kaufmann
Curr Opin Immunol. 2017 Aug;47:8-16. doi: 10.1016/j.coi.2017.06.007. Epub 2017 Jul 15.

The Cross-Species Mycobacterial Growth Inhibition Assay (MGIA) Project, 2010-2014.
Brennan MJ, Tanner R, Morris S, Scriba TJ, Achkar JM, Zelmer A, Hokey DA, Izzo A, Sharpe S, Williams A, Penn-Nicholson A, Erasmus M, Stylianou E, Hoft DF, McShane H, Fletcher HA
Clin Vaccine Immunol. 2017 Sep 5;24(9). pii: e00142-17. doi: 10.1128/CVI.00142-17

Optimatisation of a murine splenocyte mycobacterial growth inhibition assay using virulent Mycobacterium tuberculosis
Christina Jensen, Line Lindebo Holm, Erik Svensson, Claus Aagaard & Morten Ruhwald
Nature Communications volume8, Article number: 16085 (2017)  doi:10.1038/ncomms16085

Reactogenicity to major tuberculosis antigens absent in BCG is linked to improved protection against Mycobacterium tuberculosis.
Nacho Aguilo, Jesus Gonzalo-Asensio, Samuel Alvarez-Arguedas, Dessislava Marinova, Ana Belen Gomez, Santiago Uranga, Ralf Spallek, Mahavir Singh, Regine Audran, Francois Spertini & Carlos Martin

Variable BCG efficacy in rhesus populations: Pulmonary BCG provides protection where standard intra-dermal vaccination fails
Frank A.W. Verreck, Elma Z. Tchilian, Richard A.W. Vervenne, Claudia C. Sombroek, Ivanela Kondova, Okke A. Eissen, Vinod Sommandas, Nicole M. van der Werff, Ernst Verschoor, Gerco Braskamp, Jaco Bakker, Jan A.M. Langermans, Peter J. Heidt, Tom H.M. Ottenhoff, Klaas W. van Kralingen, Alan W. Thomas, Peter C.L. Beverley, Clemens H.M. Kocken

Discovery of the type VII ESX-1 secretion needle?
Ates LS, Brosch R.
Mol Microbiol. 2017 Jan;103(1):7-12.

Mycobacterium tuberculosis Complex Exhibits Lineage-Specific Variations Affecting Protein Ductility and Epitope Recognition.
Yruela I, Contreras-Moreira B, Magalhães C, Osório NS, Gonzalo-Asensio J.
Genome Biol Evol. 2017 Jan 6.

Human and Mouse Hematopoietic Stem Cells Are a Depot for Dormant Mycobacterium tuberculosis.
Tornack J, Reece ST, Bauer WM, Vogelzang A, Bandermann S, Zedler U, Stingl G, Kaufmann SH, Melchers F.
PLoS One. 2017 Jan 3;12(1):e0169119. doi: 10.1371/journal.pone.0169119. eCollection 2017.

Publications 2016

Lack of Response to HBHA in HIV-Infected Patients with Latent Tuberculosis Infection.
Delogu G, Vanini V, Cuzzi G, Chiacchio T, De Maio F, Battah B, Pinnetti C, Sampaolesi A, Antinori A, Goletti D.
Scand J Immunol. 2016 Dec;84(6):344-352. doi: 10.1111/sji.12493.

C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells
Anthony Troegelera, Ingrid Merciera, Céline Cougoulea, Danilo Pietrettia, André Coloma, Carine Duvala, Thien-Phong Vu Manhc, Florence Capillad, Renaud Poinclouxa, Karine Pingrisa, Jérôme Nigoua, Jörg Rademanne, Marc Dalodc, Frank A. W. Verreckf, Talal Al Saatid, Geanncarlo Lugo-Villarinoa, Bernd Lepeniesg, Denis Hudrisiera and Olivier Neyrollesa
PNAS Dec 2016, doi: 10.1073/pnas.1613254114

Correlates of tuberculosis risk: predictive biomarkers for progression to active tuberculosis.
Petruccioli E, Scriba TJ, Petrone L, Hatherill M, Cirillo DM, Joosten SA, Ottenhoff TH, Denkinger CM, Goletti D.
Eur Respir J. 2016 Dec;48(6):1751-1763. 

High-throughput and computational approaches for diagnostic and prognostic host tuberculosis biomarkers.
Weiner J, Kaufmann SHE
Journal of Infectious Diseases (2016), DOI:

Novel approaches to TB vaccine development.
Stefan H.E. Kaufmanna, ,January Weinera, C. Fordham von Reynb
International Journal of Infectious Diseases Nov 2016. DOI:

Lipase Processing of Complex Lipid Antigens.
Sander P, Becker K, Molin MD.
Cell Chem Biol. 2016 Sep 22;23(9):1044-6.

Inflammatory Properties and Adjuvant Potential of Synthetic Glycolipids Homologous to Mycolate Esters of the Cell Wall of Mycobacterium tuberculosis.
Tima HG, Al Dulayymi JR, Denis O, Lehebel P, Baols KS, Mohammed MO, L’Homme L, Sahb MM, Potemberg G, Legrand S, Lang R, Beyaert R, Piette J, Baird MS, Huygen K, Romano M.
J Innate Immun. 2016 Nov 18.

Microfluidics based manufacture of liposomes simultaneously entrapping hydrophilic and lipophilic drugs.
Joshi, Sameer and Hussain, Maryam T and Roces, Carla B and Anderluzzi, Giulia and Kastner, Elisabeth and Salmaso, Stefano and Kirby, Daniel J and Perrie, Yvonne.
International Journal of Pharmaceutics, 2016, ISSN 0378-5173

Rapid quantification and validation of lipid concentrations within liposomes.
Carla B Roces, Elisabeth Kastner, Peter Stone , Deborah Lowry and Yvonne Perrie.
Pharmaceutics 2016, 8(3), 29

Type VII Secretion Systems in Gram-Positive Bacteria.
Daria Bottai, Matthias I. Gröschel, Roland Brosch
“Protein Export in Gram-positive Bacteria”, within the book series  “Current Topics in Microbiology and Immunology” (CTMI), Springer;
Chapter, Part of the series Current Topics in Microbiology and Immunology pp 1-31, 2016

Sleeping Beauty and the Story of the Bacille Calmette-Guérin Vaccine.
Fletcher HA.
MBio. 2016 Aug 30;7(4). pii: e01370-16.

Mycobacterium tuberculosis components expressed during chronic infection of the lung contribute to long-term control of pulmonary tuberculosis in mice.
Claudio Counoupas, Rachel Pinto, Gayathri Nagalingam, Grant A Hill-Cawthorne, Carl G Feng, Warwick J Britton & James A Triccas.
npj Vaccines 1, Article number: 16012 (2016)

Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria.
Boritsch EC, Khanna V, Pawlik A, Honoré N, Navas VH, Ma L, Bouchier C, Seemann T, Supply P, Stinear TP, Brosch R.
Proc Natl Acad Sci U S A. 2016 Aug 30;113(35):9876-81.

ESX secretion systems: mycobacterial evolution to counter host immunity.
Gröschel MI, Sayes F, Simeone R, Majlessi L, Brosch R.
Nat Rev Microbiol. 2016 Nov;14(11):677-691.

Mycobacterium tuberculosis lipoproteins in virulence and immunity – fighting with a double-edged sword.
Becker K, Sander P.
FEBS Lett, 590: 3800–3819.

TB biomarkers, TB correlates and human challenge models: New tools for improving assessment of new TB vaccines.
Kaufmann SH, Fortune S, Pepponi I, Ruhwald M, Schrager LK, Ottenhoff TH.
Tuberculosis (Edinb). 2016 Aug;99 Suppl 1:S8-S11

CD4+ T Cells Recognizing PE/PPE Antigens Directly or via Cross Reactivity Are Protective against Pulmonary Mycobacterium tuberculosis Infection.
Sayes F, Pawlik A, Frigui W, Gröschel MI, Crommelynck S, Fayolle C, Cia F, Bancroft GJ, Bottai D, Leclerc C, Brosch R, Majlessi L.
PLoS Pathog. 2016 Jul 28;12(7):e1005770.

Innate signaling by mycobacterial cell wall components and relevance for development of adjuvants for subunit vaccines.
Tima HG, Huygen K, Romano M.
Expert Rev Vaccines. 2016 Nov;15(11):1409-1420.

Use of several immunological markers to model the probability of active tuberculosis.
Petruccioli E, Navarra A, Petrone L, Vanini V, Cuzzi G, Gualano G, Palmieri F, Girardi E, Goletti D.
Diagn Microbiol Infect Dis. 2016 Oct;86(2):169-71

A Mouse Model of Latent Tuberculosis Infection to Study Intervention Strategies to Prevent Reactivation.
Andreas Kupz , Ulrike Zedler, Manuela Stäber, Stefan H. E. Kaufmann.
PLoS One. 2016; 11(7): e0158849.

Syndecans promote mycobacterial internalization by lung epithelial cells.
Zimmermann N, Saiga H, Houthuys E, Moura-Alves P, Koehler A, Bandermann S, Dorhoi A, Kaufmann SH.
Cell Microbiol. 2016 Jun 9.

Tuberculosis Biomarkers: From Diagnosis to Protection.
Delia Goletti, Elisa Petruccioli, Simone A. Joosten, and Tom H.M. Ottenhoff.
Infect Dis Rep. 2016 Jun 24; 8(2): 6568.

Patients with Tuberculosis Have a Dysfunctional Circulating B-Cell Compartment, Which Normalizes following Successful Treatment.
Joosten SA, van Meijgaarden KE, Del Nonno F, Baiocchini A, Petrone L, Vanini V, Smits HH, Palmieri F, Goletti D, Ottenhoff TH.
PLoS Pathog. 2016 Jun 15;12(6):e1005687.

Granzyme A Is Expressed in Mouse Lungs during Mycobacterium tuberculosis Infection but Does Not Contribute to Protection In Vivo.
Uranga S, Marinova D, Martin C, Pardo J, Aguilo N.
PLoS One. 2016 Apr 7;11(4):e0153028.

Deletion of nuoG from the Vaccine Candidate Mycobacterium bovis BCG ΔureC::hly Improves Protection against Tuberculosis.
Gengenbacher 1, Nieuwenhuizen N, Vogelzang A, Liu H, Kaiser P, Schuerer S, Lazar D, Wagner I, Mollenkopf HJ, Kaufmann SH.
MBio. 2016 May 24;7(3). pii: e00679-16

Perspectives on mycobacterial vacuole-to-cytosol translocation: the importance of cytosolic access.
Simeone R, Majlessi L, Enninga J, Brosch R.
Cell Microbiol. 2016 Aug;18(8):1070-7.

Vaccination against tuberculosis with whole cell 1 mycobacterial vaccines.
Thomas J. Scriba, Stefan H.E. Kaufmann, Paul Henri Lambert, Melvin Sanicas, Carlos Martin, Olivier Neyrolles.
J Infect Dis. 2016 Sep 1;214(5):659-64.

Innate signaling by mycobacterial cell wall components and relevance for development of adjuvants for subunit vaccines.
Tima HG, Huygen K, Romano M.
Expert Rev Vaccines. 2016 May 30:1-12. [Epub ahead of print]

Post-exposure vaccination with the vaccine candidate Bacillus Calmette–Guérin ΔureC::hly induces superior protection in a mouse model of subclinical tuberculosis
Martin Gengenbacher Peggy Kaiser, Stefanie Schuerer, Doris Lazar, Stefan H.E. Kaufmann
Microbes Infect 2016 May 17;18(5):364-8. Epub 2016 Mar 17. doi:10.1016/j.micinf.2016.03.005

Vaccination in early life: standing up to the challenges
Elodie Mohr, Claire-Anne Siegrist
Opinion in Immunology, Volume 41, August 2016, Pages 1–8 doi:10.1016/j.coi.2016.04.004

Granzyme A Is Expressed in Mouse Lungs during Mycobacterium tuberculosis Infection but Does Not Contribute to Protection In Vivo
Santiago Uranga, Dessislava Marinova, Carlos Martin, Julián Pardo, Nacho Aguilo
PLOS, April 7, 2016

BCG Vaccination Induces Robust CD4+ T Cell Responses to Mycobacterium tuberculosis Complex-Specific Lipopeptides in Guinea Pigs.
Kaufmann E, Spohr C, Battenfeld S, De Paepe D, Holzhauser T, Balks E, Homolka S, Reiling N, Gilleron M, Bastian M.
J Immunol. 2016 Mar 15;196(6):2723-32. doi: 10.4049/jimmunol.1502307. Epub 2016 Feb 17.

The BCG Strain Pool: Diversity Matters.
Bottai D, Brosch R.
Mol Ther. 2016 Feb;24(2):201-3. doi: 10.1038/mt.2016.18.

Lipoarabinomannan-responsive Polycytotoxic T Cells are Associated With Protection in Human Tuberculosis.
Busch M, Herzmann C, Kallert S, Zimmermann A, Höfer C, Mayer D, Zenk SF, Muche R, Lange C, Bloom BR, Modlin RL, Stenger S; TBornotTB Network.
Am J Respir Crit Care Med. 2016 Feb 16. [Epub ahead of print]

Towards new TB vaccines: what are the challenges?
Dockrell HM.
Pathog Dis. 2016 Mar 9. pii: ftw016. [Epub ahead of print]

Molecular Determinants in Phagocyte-Bacteria Interactions.
Kaufmann SH, Dorhoi A.
Immunity. 2016 Mar 15;44(3):476-91. doi: 10.1016/j.immuni.2016.02.014.

Pathology and immune reactivity: understanding multidimensionality in pulmonary tuberculosis.
Dorhoi A, Kaufmann SH.
Semin Immunopathol. 2016 Mar;38(2):153-66. doi: 10.1007/s00281-015-0531-3. Epub 2015 Oct 5.

On the impact of masking and blocking hypotheses for measuring the efficacy of new tuberculosis vaccines.
Arregui S, Sanz J, Marinova D, Martín C, Moreno Y.
PeerJ. 2016 Feb 11;4:e1513. doi: 10.7717/peerj.1513. eCollection 2016.

Reprogramming the T Cell Response to Tuberculosis
Joshua S. Woodworth, Peter Andersen
Cell, DOI:

Mycobacterial pan-genome analysis suggests important role of plasmids in the radiation of type VII secretion systems.
Dumas E, Boritsch EC, Vandenbogaert M, Rodríguez de la Vega RC, Thiberge JM, Caro V, Gaillard JL, Heym B, Girard-Misguich F, Brosch R, Sapriel G.
Genome Biol Evol. 2016 Jan 8. pii: evw001.

Inflammation and the coagulation system in tuberculosis: Tissue Factor leads the dance.
Caccamo N, Dieli F.
Eur J Immunol. 2016 Jan 14. doi: 10.1002/eji.201546225.

Publications 2015

Pathology and immune reactivity: understanding multidimensionality in pulmonary tuberculosis.
Dorhoi A, Kaufmann SH.
Semin Immunopathol. 2015 Oct 5.

“Protein Export in Gram-positive Bacteria”, within the book series “Current Topics in Microbiology and Immunology” (CTMI), Springer;
Volume editors: Fabio Bagnoli and Rino Rappuoli
Chapter: Type VII secretion systems in Gram-positive bacteria
Daria Bottai, Matthias I. Gröschel, and Roland Brosch

Detection of IgG1 antibodies against Mycobacterium tuberculosis DosR and Rpf antigens in tuberculosis patients before and after chemotherapy
Ana Márcia Menezes Mattos, Alexandre Silva Chaves, Kees L.M.C. Franke, Bárbara Bruna Muniz Figueiredo, Ana Paula Ferreira, Tom H.M. Ottenhoff, Henrique Couto Teixeira
Tuberculosis , Volume 96 , 65 – 70, DOI:

Analysis of MHC immunopeptidomes using mass spectrometry.
Caron E, Kowalewski DJ, Koh CC, Sturm T, Schuster H, Aebersold R.
Mol Cell Proteomics. 2015 Oct 19. pii: mcp.O115.052431. [Epub ahead of print]

Versatile myeloid cell subsets contribute to tuberculosis-associated inflammation.
Dorhoi A, Kaufmann SH.
Eur J Immunol. 2015 Aug;45(8):2191-202.

An open-source computational and data resource to analyze digital maps of immunopeptidomes.
Caron E, Espona L, Kowalewski DJ, Schuster H, Ternette N, Alpízar A, Schittenhelm RB, Ramarathinam SH, Lindestam Arlehamn CS, Chiek Koh C, Gillet LC, Rabsteyn A, Navarro P, Kim S, Lam H, Sturm T, Marcilla M, Sette A, Campbell DS, Deutsch EW, Moritz RL, Purcell AW, Rammensee HG, Stevanovic S, Aebersold R.
Elife. 2015 Jul 8;4. doi: 10.7554/eLife.07661.

Evolutionary Landscape of the Mycobacterium tuberculosis Complex from the Viewpoint of PhoPR: Implications for Virulence Regulation and Application to Vaccine Development
Esther Broseta, Carlos Martín, Jesús Gonzalo-Asensio
doi: 10.1128/mBio.01289-15 20 October 2015 mBio vol. 6 no. 5 e01289-15

MTBVAC vaccine is safe, immunogenic and confers protective efficacy against Mycobacterium tuberculosis in newborn mice
Nacho Aguilo, Santiago Uranga, Dessislava Marinova, Marta Monzon, Juan Badiola, Carlos Martin

Pulmonary but Not Subcutaneous Delivery of BCG Vaccine Confers Protection to Tuberculosis-Susceptible Mice by an Interleukin 17-Dependent Mechanism.
Aguilo N, Alvarez-Arguedas S, Uranga S, Marinova D, Monzón M, Badiola J, Martin C.
J Infect Dis. 2015 Oct 22. pii: jiv503. [Epub ahead of print]

Immunological Signatures Identifying Different Stages of Latent Mycobacterium tuberculosis Infection and Discriminating Latent from Active Tuberculosis in Humans.
Smits K, Corbière V, Dirix V, Mekkaoui L, Wyndham-Thomas C, et al. (2015)
J Clin Cell Immunol 6:341. doi:10.4172/2155-9899.1000341

Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers
Maria M. Esterhuyse, January Weiner 3rd, Etienne Caron, Andre G. Loxton, Marco Iannaccone, Chandre Wagman, Philippe Saikali, Kim Stanley, Witold E. Wolski, Hans-Joachim Mollenkopf, Matthias Schick, Ruedi Aebersold, Heinz Linhart, Gerhard Walzl, Stefan H. E. Kaufmann
doi: 10.1128/mBio.01187-15, 15 September 2015 mBio vol. 6 no. 5 e01187-15

Synthetic Long Peptide Derived from Mycobacterium tuberculosis Latency Antigen Rv1733c Protects against Tuberculosis.
Coppola M, van den Eeden SJ, Wilson L, Franken KL, Ottenhoff TH, Geluk A.
Clin Vaccine Immunol. 2015 Sep;22(9):1060-9. doi: 10.1128/CVI.00271-15. Epub 2015 Jul 22.

Molecular signatures for vaccine development.
Maertzdorf J, Kaufmann SH, Weiner J 3rd.
Vaccine. 2015 Apr 6. pii: S0264-410X(15)00394-1. doi: 10.1016/j.vaccine.2015.03.075. [Epub ahead of print]

Protein energy malnutrition during vaccination has limited influence on vaccine efficacy but abolishes immunity if administered during Mycobacterium tuberculosis infection.
Hoang T, Agger EM, Cassidy JP, Christensen JP, Andersen P.
Infect Immun. 2015 Mar 9. pii: IAI.03030-14. [Epub ahead of print]

Liposomal delivery of lipoarabinomannan triggers Mycobacterium tuberculosis specific T-cells.
Kallert s, Zenk SF, Walther P, Grieshober M, Weil T, Stenger S.
Tuberculosis (Edinb). 2015 Jul;95(4):452-62. doi: 10.1016/ Epub 2015 May 19.

Molecular signatures for vaccine development.
Maertzdorf J, Kaufmann SH, Weiner J 3rd.
Vaccine. 2015 Apr 6. pii: S0264-410X(15)00394-1. doi: 10.1016/j.vaccine.2015.03.075. [Epub ahead of print]

High-throughput data analysis and data integration for vaccine trials.
Weiner J 3rd, Kaufmann SH, Maertzdorf J.
Vaccine. 2015 May 11. pii: S0264-410X(15)00593-9. doi: 10.1016/j.vaccine.2015.04.096. [Epub ahead of print]