Scientific publications
Publications 2010
Liposomal cationic charge and antigen adsorption are important properties for the efficient deposition of antigen at the injection site and immunogenicity of the vaccine.
Journal of Controlled release. Accepted, March 2010.
Malou Henriksen-Lacey, Vincent W. Bramwell, Dennis Christensen, Else-Marie Agger, Peter Andersen, Yvonne Perrie (2010).
The second Geneva Consensus: Recommendations for novel live TB vaccines.
Vaccine. 2010 Mar 8;28(11):2259-70.
Walker KB, Brennan MJ, Ho MM, Eskola J, Thiry G, Sadoff J, Dobbelaer R, Grode L, Liu MA, Fruth U, Lambert PH.
HBHA-specific IFN-g synthesis at the site of infection during active tuberculosis in humans.
American Journal of Respiratory and Critical Care Medicine 2010
Place S., Verscheure V., de San N., Hougardy J.M., Schepers K., Dirix V., Dediste A., Michel O., Drowart A., Allard S., Doherty M, Lecher S., Locht C., Mascart F
Polyfunctional T cells in human tuberculosis
Eur. J. Immunol. 2010. 40: 2139–2142
Katalin A. Wilkinson and Robert J. Wilkinson
Partial and Ineffective Activation of Vγ9Vδ2 T Cells by Mycobacterium tuberculosis-Infected Dendritic Cells
The Journal of Immunology, June 30, 2010, 1000966
Serena Meraviglia, Nadia Caccamo, Alfredo Salerno, Guido Sireci and Francesco Dieli
Multifunctional CD41 T cells correlate with active Mycobacterium tuberculosis infection
Eur. J. Immunol. 2010. 40: 2211–2220
Nadia Caccamo, Giuliana Guggino, Simone A. Joosten, Giuseppe Gelsomino, Paola Di Carlo, Lucina Titone, Domenico Galati,Marialuisa Bocchino, Alessandro Matarese4, Alfredo Salerno,Alessandro Sanduzzi, Willeke P. J. Franken, Tom H. M. Ottenhoff and Francesco Dieli
Publications 2009
Immunogenicity and protective efficacy of prime-boost regimens with recombinant ureC hly+ Mycobacterium bovis BCG and modified vaccinia virus Ankara expressing M. tuberculosis antigen 85A against murine tuberculosis.
Infect. Immun. 77: 622-631 (2009)
Tchilian E.Z., C. Desel, E.K. Forbes, S. Bandermann, C. Sander, A.V. Hill, H. McShane, S.H.E. Kaufmann
Intracellular replication of attenuated Mycobacterium tuberculosis phoP mutant in the absence of host cell cytotoxicity.
Microbes Infect 11: 115-122.
Ferrer, N.L., Gomez, A.B., Soto, C.Y., Neyrolles, O., Gicquel, B., Portillo, F.G., and Martin, C. (2009)
Extended safety studies of the attenuated live tuberculosis vaccine SO2 based on phoP mutant.
Vaccine 27: 2499-2505. (2009)
Cardona, P.J., Asensio, J.G., Arbues, A., Otal, I., Lafoz, C., Gil, O., Caceres, N., Ausina, V., Gicquel, B., and Martin, C.
MVA.85A boosting of BCG and an attenuated, phoP deficient M. tuberculosis vaccine both show protective efficacy against tuberculosis in rhesus macaques.
PLoS One 4: e5264 (2009)
Verreck, F.A., Vervenne, R.A., Kondova, I., van Kralingen, K.W., Remarque, E.J., Braskamp, G., van der Werff, N.M., Kersbergen, A., Ottenhoff, T.H., Heidt, P.J., Gilbert, S.C., Gicquel, B., Hill, A.V., Martin, C., McShane, H., and Thomas, A.W.
Identification of Major Factors Influencing ELISpot-Based Monitoring of Cellular Responses to Antigens from Mycobacterium tuberculosis.
PLoS ONE 4(11): e7972. (2009)
Smith SG, Joosten SA, Verscheure V, Pathan AA, McShane H, et al.
Immunological memory transferred with CD4 T cells specific for tuberculosis antigens Ag85B-TB10.4: persisting antigen enhances protection.
PloS ONE 4(12):e8272, 2009
Duffy D, Dawoodji A, Agger EM, Andersen P, Westermann J, Bell EB
Liposomes based on dimethyldioctadecylammonium promote a depot effect and enhance immunogenicity of soluble antigen.
J Control Release, 2009
Henriksen-Lacey M, Bramwell VW, Christensen D, Agger EM, Andersen P, Perrie Y.
Novel generation mycobacterial adjuvant based on liposome encapsulated monomycolyl glycerol from Mycobacterium bovis BCG.
JI 183(4):2294-302, 2009.
Andersen CS, Rosenkrands I, Olsen AW, Nordly P, Christensen D, Lang R, Kirschning C, Gomes JM, Bhowruth V, Minnikin DE, Besra GS, Follmann F, Andersen P, Agger EM.
A liposome-based mycobacterial vaccine induces potent adult and neonatal multifunctional T cells through the exquisite targeting of dendritic cells.
PloS ONE 4(6): e5771, 2009.
Kamath A, Rochat AF, Christensen D, Agger EM, Andersen P, Lambert PH, Siegrist CA.
Tuberculosis subunit vaccination provides long-term protective immunity characterized by multifunctional CD4 memory T cells.
JI 182:8047-55, 2009.
Lindenstrøm T, Agger EM, Korsholm KS, Darrah PA, Aagaard C, Seder RA, Rosenkrands I, Andersen P.
Adjuvant properties of a simplified C32 monomycolyl glycerol.
Bioorg Med Chem Lett. 19:2029-32, 2009.
Bhowruth V, Minnikin DE, Agger EM, Andersen P, Bramwell VW, Perrie Y, Besra GS.
Adjuvanticity of a synthetic cord factor analogue for subunit Mycobacterium tuberculosis vaccination requires FcR –Syk–Card9–dependent innate immune activation.
J. Exp. Med. 206:89-97, 2009.
Werninghaus K, Babiak A, Groß O, Hölscher C, Dietrich H, Agger EM, Mages J, Mocsai A, Schoenen H, Finger K, Nimmerjahn F, Brown GD, Kirschning C, Heit A, Andersen P, Wagner H, Ruland J, Lang R.
A simple mycobacterial monomycolated glycerol lipid has potent immunostimulatory activity.
J of Immunol. 182(1):424-32, 2009 (shared first authorship).
Andersen CS, Agger EM, Rosenkrands I, Gomes JM, Bhowruth V, Gibson KJ, Petersen RV, Minnikin DE, Besra GS, Andersen P.
Subcutaneous boosting with heparin-binding haemagglutinin increases BCG-induced protection against tuberculosis.
Microbes Infect. 11, 995-1001 2009.
Rouanet, C., Debrie, A.-S., Lecher, S., Locht, C.
A murine DC-SIGN homologue contributes to early host defense against Mycobacterium tuberculosis.
J Exp Med. 2009 Sep 28;206(10):2205-20.
Tanne A, Ma B, Boudou F, Tailleux L, Botella H, Badell E, Levillain F, Taylor ME, Drickamer K, Nigou J, Dobos KM, Puzo G, Vestweber D, Wild MK, Marcinko M, Sobieszczuk P, Stewart L, Lebus D, Gicquel B, Neyrolles O
Response to Rv2628 latency antigen associates with cured tuberculosis and remote infection.
Eur Respir J. 2009 Nov 19.
Goletti D, Butera O, Vanini V, Lauria FN, Lange C, Franken KL, Angeletti C, Ottenhoff TH, Girardi E.
Cross-reactive immunity to Mycobacterium tuberculosis DosR regulon-encoded antigens in individuals infected with environmental, nontuberculous mycobacteria.
Infect Immun. 2009 Nov;77(11):5071-9.
Lin MY, Reddy TB, Arend SM, Friggen AH, Franken KL, van Meijgaarden KE, Verduyn MJ, Schoolnik GK, Klein MR, Ottenhoff TH
Overcoming the global crisis: "yes, we can", but also for TB ... ?
Eur J Immunol. 2009 Aug;39(8):2014-20.
Ottenhoff TH.
Analysis of Mycobacterium tuberculosis-specific CD8 T-cells in patients with active tuberculosis and in individuals with latent infection
PLoS One. 2009;4(5):e5528. Epub 2009 May 13.PMID: 19436760
Caccamo N, Guggino G, Meraviglia S, Gelsomino G, Di Carlo P, Titone L, Bocchino M, Galati D, Matarese A, Nouta J, Klein MR, Salerno A, Sanduzzi A, Dieli F, Ottenhoff TH
Mycobacterium tuberculosis peptides presented by HLA-E molecules are targets for human CD8 T-cells with cytotoxic as well as regulatory activity.
PLoS Pathog. 2010 Feb 26;6(2):e1000782.
Joosten SA, van Meijgaarden KE, van Weeren PC, Kazi F, Geluk A, Savage ND, Drijfhout JW, Flower DR, Hanekom WA, Klein MR, Ottenhoff TH.
Liposome-based cationic adjuvant formulations (CAF): Past, present, and future.
Journal of Liposome Research. 19(1) 2-11.
Christensen D, Agger EM, Andreasen LV, Kirby, DJ, Andersen P, Perrie Y (2009).
Mycolic acids constitute a scaffold for mycobacterial lipid antigens stimulating CD1-resticted T cells.
Chem Biol. 30: 82-92; 2009
Layre E., Collmann A, Bastian M, Mariotti S, Czaplicki J, Prandi J, Mori L, Stenger S, De Libero G, Puzo G, Gilleron M.
Anti-TNF immunotherapy reduces CD8+ T cell-mediated antimicrobial activity against Mycobacterium tuberculosis.
J. Clin. Invest. 119: 1167-1177, 2009
Bruns H, Meinken C, Schauenberg P, Härter G, Kern P, Modlin R.L, Antoni C, Stenger S.
Publications 2008
Claire S Andersen, Else-Marie Agger, Ida Rosenkrands, Jessica M. Gomest, Veemal Bhowruth, Kevin JC Gibson, Rune V. Petersen, David E. Minnikin, Gurdyal S. Besra, Peter Andersen.
A simple mycobacterial monomycolated glycerol lipid has potent immunostimulatory activity.
J. Immunol. 182(1); 424-32 (2009)
Else-Marie Agger, Ida Rosenkrands, Jon Hansen, Karima Brahimi, Brian S Vandahl, Claus Aagaard, Kerstin Werninghaus, Carsten Kirschning, Roland Lang, Dennis Christensen, Michael Theisen, Frank Follmann and Peter Andersen.
Cationic liposomes formulated with synthetic mycobacterial cordfactor (CAF01); a versatile adjuvant for vaccines with different immunological requirement.
PLoS ONE
Julie Guiard, Anthony Collman, Martine Gilleron, Lucia Mori, Gennaro De Libero, Jacques Prandi, German Puzo.
Title: Synthesis of diacylated trehalose sulfate, analogs of a mycobacterial sulfoglycolipid antigen and candidates for a tuberculosis vaccine.
Wiley InterScience; 3Nov 2008, vol.120 (50) 9880-9884
Julie Guiard, Anthony Collmann, Louis-Fernando Garcia-Alles, Lionel Mourey, Thérèse Brando, Lucia Mori, Martine Gilleron, Jacques Prandi, Gennaro De Libero, Germain Puzo
Fatty acyl structures of Mycobacterium tuberculosis sulfoglycolipid govern T cell response.
J. Immunol. 2009 Jun 1;182(11): 7030-7
Pascale Peyron, Julien Vaubourgeix, Yannick Poquet, Florence Levillain, Catharine Botanch, Fabienne Bardou, Mamadou Daffé, Jean-Francois Emile, Bruno Marchou, Pere-Joan Cardona, Chantal de Chastellier, Frédéric Altare.
Foamy Macophages fro Tuberculous Patients Granulomas Constritute a Nutrient Rich Reservoir for M.tuberculosis Persistence.
PLoS Pathogens Nov. 2008
Julie Guiard, Anthony Collmann, Martine Gilleron, Lucia Mori, Gennaro De Libero, Jacques Prandi and Germain Puzo.
Synthesis of Diacylated Trehalose Sulfates: Candidates for a Tuberculosis Vaccine.
Wiley InterScience, 3 Nov. 2008; vol 120 (50) 9880-9884
Emilie Layre, Anthony Collmann, Max Bastian, Sabrina Mariotti, Jerzy Czaplicki, Jacques Prandi, Lucia Mori, Steffen Stenger, Gennaro De Libero, Germain Puzo and Martine Gilleron.
Mycolic Acids Constitute a Scaffold for Mycobaterial Lipid Antigens Stimulating CD1-Restricted T Cells.
Cell Press Chemistry & Biology Article Vol 16, (1) 30 Jan. 2009; 82-92
Max Bastian, Tobias Braun, Heiko Bruns, Martin Röllinghof and Steffen Stenger.
Mycobacterial Lipopeptides Elicit CD4+ CTLs in Mycbactrium tuberculosis-Infected Humans
The Journal of Immunology: 2008, 180, 3436-3446
Jong Seok Lee, Roland Krause, Jörg Schreiber, Hans-Joachim Mollenkopf, Jane Kowall, Robert Stein, Bo-Young Jeon, Jeong-Yeon Kak, Min-Kyong Song, Juan Pablo Patron, Sabine Jorg, Kyoungmin Roh, Sang-Nae Cho and Stefan Kaufmann.
Mutation in the Transcriptional Regulator PhoP Contributes to Avirulence of Mycobacterium tuberculosis H37Ra Strain.
Cell Host & Microbe Vol. 3, Issue 2, 14 FEbr. 2008, 97-103
Nadia Caccamo, Giuliana Guggino, Serena Meraviglia, Giuseppe Gelsomino, Paolo Di Carlo, Lucina Titone, Marialuisa Bocchino, Domenico Galati, Allessandro Matarese, Jan, Nouta, Michel Klein, Alfredo Salerno, Allessandro Sandussi, Frencesco Dieli and Tom Ottenhoff.
Analysis of Mycobacterium tuberculosis-specific CD8 T-cells with active tuberculosis and in individuals with latent infection
Journal List>PLosOne> v. 4(5); 2009 e5528
Pere J. Cardona, Jesus A Gonzalo Asensio, Ainhoa Arbues, Isabel Otal, Carmen Lafoz, Olga Gil, Neus Caceres, Vicente Ausina, Brigitte Gicquel Carlos Martin.
Extended safety studies of the attenueated live tuberculosis vaccine S)2 based on phoP mutant.
Vaccine vol. 27, Issue 18. 21 April 2009, 2499-2505
Nadia L. Ferrer, Ana B Gómez, Carlos Y Soto, Olivier Neyrolles, Brigitte Gicquel, Francisco Garcia-del Portillo and Carlos Martin.
Intracellular replication of attenuated Mycobacterium tuberculosis phoP mutant in the absence of host cell cytotoxicity.
Microbes and Infection, Vol. 11, Issue 1, Jan. 2009 115-122
Jesus Gonzalo-Asensio, Serge Mostowy, Jose-Harders-Westerveen, Kris Huygen, Rogelio Hernández-Pando, Jelle Thole, Marcel Behr, Brigitte Gicquel and Carlos Martin.
PhoP: a Missing Piece in the Intricate Puzzle of Mycobacterium tuberculosis Virulence.
PLosOne, 2008;3(10):e3496 Epub 2008 Oct.23
Jesus Gonzalo-Asensio, Carlos Y. Soto, Ainhoa Arbués, Javier Sancho, Maria del Carmen Menéndez, Maria J. Garcia, Brigitte Gicquel and Carlos Martin.
Mycobacterium tuberculosis phoPR operon is positively autoregulated in the virulent strain H37Rv
J Bacteriol. 2008 Nov; 190(21); 7068-78 Epub 2008 Aug. 29
Arun T Kamath, Anne-Francoise Rochat, Dennis Christenses, Else Marie Agger, Peter Andersen, Paul-Henri Lambert, Claire-Anne Siegrist.
A liposome-based mycobacterial vaccine induces potent adult and neonatal multifunctional T cells through the exquisite targeting of dendritic cells.
PLosOne, 2009 Jun 3;4(6):e5771
Arun T Kamath, Anne-Francoise Rochat, Mario P. Valenti, Else-Marie Agger, Laren Lingnau, Peter Andersen, Paul-Henri Lambert, Claire-Anne Siegrist
Adult-like Anti-Mycobacterial T Cell and in vivo Dendritic Cell Responses following Neonatal Immunization with Ag85B-ESAT-6 in the IC31 Adjuvant
PLosOne 2008;3(11):e3683, Epub 2008 Nov. 10
Tom H.M. Ottenhoff
Overcoming the global crisis “Yes, we can”, but also for TB…?
Wiley InterScience, Vol 39, Issue 8; 2014-2020
Maytal Bivas-Benita, May-Young Lin, Suzanne Bal, Krista E. van Meijgaarden, Kees L.M.C. Franken, Annemieke H. Friggen, Hans E. Junginger, Gerrit Borchard, Michèl R. Klein and Tom H.M. Ottenhoff.
Pulmonary delivery of DNA encoding Mycobacterium tuberculosis latency antigen Rv1733c associated to PLGA-PEI nanoparticles enhances T cell responses in a DNA prime/protein boost vaccination regimen in mice.
Vaccine 2009 Jun 19;27(30): 4010-7
Franck A.W. Verreck, Richard A.W. Vervenne, Ivanela Kondova, Klaas W. van Kralingen, Ed Remarque, Gerco Brascamp, Nicole M. van der Werff, Ariena Kersbergen, Tom H.M. Ottenhoff, Peter J. Heidt, Sarah C. Gilbert, Brigitte Gicquell, Adrian V.S. Hill, Carlos Martin, Helen McShane, Alan W. Thomas.
MVA.85A Boosting of BCG and an Attenuated phoP Deficient M.tuberculosis Vaccine Both Show Protective Efficacy Against Tuberculosis in Rhesus Macaques.
Journal List>PLoSOne>v.4(4); 2009
Veemal Bhowruth, David E. Minnikin, Else-Marie Agger, Petr Andersen, Vincent W. Bramwell, Yvonne Perrie and Gurdyal S. Besra.
Adjuvant Properties of a Simplifid C 32 Monomycolyl Glycerol Analogue.
Bioorganic & Medicinal Chem. Letters; vol. 19, Issue 7, 1 April 2009; 2029-2032
Daniel J. Kriby, Ida Rosenkrands, Else-Marie Agger, Petr Andersen, Allan G.A. Coombes & Yvonne Perrie.
PLGA microspheres for the delivery of a novel subunit TB vaccine.
Journal of Drug Targting 2008; 00(0): 1-12
Eric B. Bell, and Jurgen Westermann
CD4 memory T cells on trial: immunological memory without a memory T cell
Trends in Immunology, Vol. 29 (9) Sept. 2008, 405-411
Helen A. Fletcher, Ansar A. Pathan, Tamara K. Berthoud, Susanna J. Dunachie, Kathryn T. Whelan, Nicola C. Alder, Clare R. Sander, Adrian V.S. Hill and Helen McShane.
Boosting BCG vaccination with MVA 85A down-regulates the immuno regulatory cytokine TGF-β1.
Vaccine 2008 September 26; 26(41):5269-5275
Natalie E.R. Beveridge, Helen A. Fletcher, Jane Hughes, Ansar A. Pathan, Thomas J. Scriba, Angela Minassian, Clare R. Sander, Kathryn T. Whelan, Hazel M. Dockrell, Adrian V.S. Hill, Willem A. Hanekom, Helen McShane.
A comparison of IFNγ detection methods used in tuberculosis vaccine trials.
Elsevier Tuberculosis (2008) 88, 631-640
Emily K. Forbes, Clare Sander, Edward O. Ronan, Helen KcShane, Adrian V.S. Hill, Peter C.L. Beverley and Elma Z. Tchilian
Multifunctiona. High Level Cytokine-Producing Th1 Cells in the Lung, but Not Spleen, Correlate with Protection against Mycobacterium tuberculosis Aerosol Challenge in Mice.
The Journal of Immunology, 2008, 181, 4955-4964
Elma Z. Tchilian, Christiane Desel, Emily K. Forbes, Silke Bandermann Clare Sander, Adria V.S. Hill, Helen McShane, and Stefan Kaufmann
Immunogenicity and Protective Efficacy of Prime-Boost Regimens with Recombinant AureC hly+ Mycobacterium bovis BCG and Modified Vaccinia Virus Ankara Expressing M.tuberculosis Antigen 85A against Murine Tuberculosis.
Infection and Immunity Feb. 2009 p. 622-631
Heiko Bruns, Christoph Meinken, Philipp Schauenberg, Georg Härter, Peter Kern, Robert L. Modlin, Christian Antoni, Steffen Stenger
Anti-TNF immunotherapy reduces CD8+T cell. Mediated antimicrobial activity against Mycobacterium tuberculosis.
J.Clin. Invest. 2009 May 1; 119(5): 1167-1177
Publications 2007
Jean-Michel Hougardy, Sammy Place, Marc Hildebrand, Annie Drowart, Anne-Sophie Debrie, Camille Locht, Françoise Mascart.
Regulatory T Cells Depress Immune Responses to Protective Antigens in Active Tuberculosis.
American Journal of Respiratory and Critical Care Medicine (2007) 409-416
Jean-Michel Hougardy, Kinda Schepers, Sammy Place, Annie Drowart,, Vérenique Lechevin, Virginie Verscheure, Anne-Sophie Debrie, T.Mark Doherty, Jean-Paul van Vooren, Camille Locht, Francoise Mascart.
Heparin-Binding-Hemagglutinin-Induced IFN-γRelease as a Diagnostic Tool for Latent Tuberculosis
PloS ONE – October (2007) Issue 10/e 926
Jean-Michel Hougardy, Virginie Verscheure, Camille Locht, Francoise Mascart
In vitro expansion of CD4+CD25highFOXP3+CD127low/- regulatory T cells from peripheral blood lymphocytes of healthy Mycobacterium tuberculosis infected humans
Elsevier Microbes and Infections 9 (2007) 1325 – 1332
Dennis Christensen, Camilla Foged, Ida Rosenkrands, Hanne Morck Nielsen, Peter Andersen, Else Marie Agger.
Trehalose preserves DDA/TDB liposomes and their adjuvant effect during freeze drying.
Elsevier Biochimica et Biophysica Acta 1768 (2007) 2120 – 2129
Else Marie Agger, Joseph P. Cassidy, Joseph Brady, Karen S. Korsholm, Carina Vingbo-Lundberg, Peter Andersen.
Adjuvant modulation of the cytokine balance in Mycobacterium tuberculosis subunit vaccines; immunity, pathology and protection.
Immunology 2008 June; 124(2): 175-185 Blackwel Publishing Ltd.
Marc Jacobsen, Dirk Repsilber, Andrea Gutschmidt, Albert Neher, Knut Feldmann, Hans J. Mollenkopf, Andreas Ziegler, and Stefan H. E. Kaufmann
Candidate biomarkers for discrimination between infection and disease caused by Mycobacterium tuberculosis.
J.Mol.Med (2007) 85:613-621
Stefan H. Kaufmann
The contribution of immonology to the rational design of novel antibacterial vaccines.
Nature Publishing Group (2007 July) 491-504
Diana Di Liberto, Simona Buccheri, Nadia Caccamo, Serena Meraviglia, Amelia Romano, Paolo Di Carlo, LLucina Titone, Francesco Dieli, Alan M. Krensky, Alfredo Salerno.
Decreased serum granulysin levels in childhood tuberculosis which reverse after therapy.
Elsevier Tuberculosis (2007) 87, 322-328
JA Gonzalo Asensio A Arbués, E. Pérez, B.Gicquel, Carlos Martin
Live tuberculosis vaccines based on phoP mutants: a step towards clinical trials.
Expert Opin. Biol. Ther. (2008) 8(2): 1-11
Simone A. Joosten, Krista E. van Meijgaarden, Nigel D.L. Savage, Tjitske de Boer, Frédéric Triebel, Annemieke van der Wal, Emile de Heer, Michèl R. Klein, Annemieke Geluk, Tom Ottenhoff.
Identification of a new human CD8+ regulatory T-cell subset which mediates suppression through the chemokine CC chemokine ligand 4
PNAS (May 2007) vol. 104, no.19 802908034
E. Sahiratmadjaa, B. Alisjahbanad, S. Buccheri, D. Di Liberto, T. de Boer, I. Adnan, R. van Crevel, M.R. Kleina, K.E. van Meijgaarden, R.H.H. Nelwan, E. van de Vosse, F. Dieli, T.H.M. Ottenhoff,
Plasma granulysin levels and cellular interferon-g production correlate with curative host responses in tuberculosis, while plasma interferon-g levels correlate with tuberculosis disease activity in adults.
Elsevier Tuberculosis (2007) 87, 312-321
Daniel J. Kirby, Ida Rosenkrands, Else Marie Agger, Peter Andersen, Allan G.A. Coombes, Yvonne Perrie,
PLGA microspheres for the delivery of a novel subunit TB vaccine.
Journal of Drug Targeting 2008; 00(0): 1-12
Helen McShane, Elma Tchilian, Adrian Hill
Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models
Elsevier Vaccine25 (2007) 8203–8205
May Young Lin, Annemieke Geluk, Ateven G. Smith, Amanda L. Stewart, Annemieke H. Frigggen, Kees L.M.C. Franken, Marleen J.C. Verduyn, Krista E. van Meijgaarden, Martin I. Voskuil, Hazel M. Dockrell, Kris Huygen, Tom H.M. Ottenhoff, Michèl R Klein
Lack of Immune Responses to Mycobacterium tuberculosis DosR Regulon Proteins following Mycobcaterium bovis BCG vaccination
Infection and Immunity, July 2007, p. 3523-3530
Max Bastian, Tobias Braun, Heiko Bruns, Martin Röllinghoff, Steffen Stenger
Mycobacterial Lipopeptides Elicit CD4+ CTLs in Mycobacterium tuberculosis-Infected Humans
The Journal of Immunology2008, 180: 3436-3446
Publications 2006
Camille Locht, Carine Rouanet, Françoise Mascart, Jean-Michel Hougardy.
Heparin-binding hemagglutinin, from an extrapulmonary dissemination factor to a powerful diagnostic and protective antigen against tuberculosis.
Elsevier. Tuberculosis (2006) 86, 303 – 309
Anil Vangala, Daniel Kirby, Ida Rosenkrands, Else Marie Agger, Peter Andersen, Yvonne Perrie
A comparative study of cationic liposome and niosome-based adjuvant systems for protein subunit vaccines: characterisation, environmental scanning electron microscopy and immunisation studies in mice.
JPP 2006, 58: 1:13
Else Marie Agger, Ida Rosenkrands, Anja Weinreich Olsen, Graham Hatch, Ann Williams, Constantia Kritsch, Karen Lingnau, Alexander von Gabain, Claire Swetman Andersen, Karen Smith Korsholm, Peter Andersen
Protective immunity to tuberculosis with Ag85B-ESAT-6 in a synthetic cationic adjuvant system IC31.
Elsevier Vaccine 24 (2006) 5452-5460.
Thomas Bennekov, Jes Dietrich, Ida Rosenkrands, Anette Stryhn, T. Mark Doherty and Peter Andersen
Alteration of epitope recognition pattern in Ag85B and ESAT-6 has a profound influence on vaccine-induced protection against Mycobacterium tuberculosis.
Eur.J. Immunol. 2006. 36: 3346-3355
Gilleron, M., B. Linder, and Puzo G.,
MS/MS Approach for Characterization of the Fatty Acid Distribution on Mycobacterial Phosphatidyl-myo-inosito lMannosides,
Anal Chem. 2006, 78(24):8543- 8548
Garcia-Alles L. F., Versluis K., Maveyraud L., Vallina A. T., Sansano S., Goeber J., Bello N. F., Guillet V., de la Salle H., Puzo G., Mori, L., HeckA., De Libero G., Mourey L.,
Endogenous phosphatidylcholine and a long spacer ligand stabilize the lipid-binding groove of CD1b,
EMBO J., 2006, 25, 3684-3692
M. Bonneville, E. Scotet
Human Vg9Vd2 T cells : promising new leads for immunotherapy of infections and tumors.
Curr Op Immunol (2006) 18 : 539 - 546
N. Caccamo, L. Battistini, M. Bonneville, F. Poccia, JJ Fournié, S. Meraviglia, G. Borsellino, R.A. Krockzek, R. La Mendola, E. Scotet, F. Dieli, A. Salerno.
CXCR5 identifies a subset of Vg9Vd2 T cells which secrete IL4 and IL10 and help B cells for antibody production.
J Immunol (2006) 177 : 5290 – 5295.
MC Devilder, S. Maillet, I. Bouyge-Moreau, E. Donnadieu, M. Bonneville, E. Scotet.
Potentiation of antigen-stimulated Vg9Vd2 T cell cytokine production by immature dendritic cells (DC) and reciprocal effect on DC maturation.
J Immunol (2006) 176 : 1386 – 1393.
Martin, C., Williams, A., Hernandez-Pando, R., Cardona, P.J., Gormley, E., Bordat, Y., Soto, C.Y., Clark, S.O., Hatch, G.J., Aguilar, D., Ausina, V., and Gicquel, B.
The live Mycobacterium tuberculosis phoP mutant strain is more attenuated than BCG and confers protective immunity against tuberculosis in mice and guinea pigs. 2006.
Vaccine 24: 3408-3419.
Gonzalo Asensio, J., Maia, C., Ferrer, N.L., Barilone, N., Laval, F., Soto, C.Y., Winter, N., Daffe, M., Gicquel, B., Martin, C., and Jackson, M.
The virulence-associated two-component PhoP-PhoR system controls the biosynthesis of polyketide-derived lipids in Mycobacterium tuberculosis. 2006.
J Biol Chem 281: 1313-1316.
Martin, C
Tuberculosis vaccines: past, present and future. 2006.
Curr Opin Pulm Med 12: 186-191
Leyten EM, Lin MY, Franken KL, Friggen AH, Prins C, van Meijgaarden KE, Voskuil MI, Weldingh K, Andersen P, Schoolnik GK, Arend SM, Ottenhoff TH, Klein MR.
Human T-cell responses to 25 novel antigens encoded by genes of the dormancy regulon of Mycobacterium tuberculosis
Microbes Infect.2006 Jul;8(8):2052-60. Epub 2006 Jun 13
E. Sahiratmadja, B. Alisjahbana, T. de Boer, I. Adnan, A. Maya, H. Danusantoso, R.H.H. Nelwan, S. Marzuki, J.W.M. van der Meer, R. van Crevel, E. van de Vosse, and T.H.M. Ottenhoff.
Dynamic Changes in Pro- and Anti-inflammatory Cytokine Profiles (IFN-γ, TNF-α, IL-12/23, and IL-10) and IFN-γReceptor Signalling Integrity Correlate with Tuberculosis Disease Activity and Response to Curative Treatment.
Infect Immun. 75:820-829 (2007) [Epub ahead of print Dec 4, 2006]
V. Roupie, M. Romano, L. Zhang, H. Korf. M.Y. Lin, K.L.Franken, T.H.M. Ottenhoff, M.R. Klein, K.Huygen.
Immunogenicity of eight dormancy (DosR) regulon encoded proteins of Mycobacterium tuberculosis in DNA vaccinated and TB infected mice.
Infect Immun. 75:941-949 (2007). [Epub ahead of print Dec 4, 2006].
Vangala A, Kirby D, Rosenkrands I, Agger EM, Andersen P, Perrie Y.
A comparative study of cationic liposome and niosome-based adjuvant systems for protein subunit vaccines: characterisation, environmental scanning electron microscopy and immunisation studies in mice.
JPP 2006, 58: 787 - 799
Mohammed AR, Bramwell VW, Coombes AG, Perrie Y.
Lyophilisation and sterilisation of liposomal vaccines to produce stable and sterile products.
Elsevier Methods 40 (2006) 30-38.
Bramwell VW, Perrie Y.
Particulate delivery systems for vaccines: what can we expect?
JPP 2006, 58: 717 - 728
Hannah B Ibanga, Roger H Brooks, Philip C Hill, Patrick K Owiage, Helen A. fletcher, Christian Lienhardt, Adrian VS Hill, Richard A. Adegbola, Helen McSchane
Early clinical trials with a new tuberculosis vaccine, MVA85A, in tuberculosis-endemic countries: issues in study design
Lancet Infect Dis 2006: 6: 522-28
Publications 2005
Ida Rosenkrands, Else Marie Agger, Anja W. Olsen, Karen S. Korsholm, Claire Swetman Andersen, Klaus T. Jensen, and Peter Andersen.
Cationic Liposomes Containing Mycobacterial Lipids: a New Powerful Th1 Adjuvant System.
Infect. Immun. 2005, 73(9): 5817-5826.
Jesper Davidsen, Ida Rosenkrands, Dennis Christensen, Anil Vangala, Daniel Kirby, Yvonne Perrie, Else Marie Agger, and Peter Andersen.
Characterization of cationic liposomes based on dimethyldioctadecylammonium and synthetic cord factor from M. tuberculosis (trehalose 6,6′-dibehenate)—A novel adjuvant inducing both strong CMI and antibody responses.
Biochim Biophys Acta 2005, 1718(1-2):22-31.
Marie-Claire Devilder, Sophie Maillet, Isabelle Bouyge-Moreau, Emmanuel Donnadieu, Marc Bonneville and Emmanuel Scotet.
Potentiation of Antigen-Stimulated V9V2 T Cell Cytokine Production by Immature Dendritic Cells (DC) and Reciprocal Effect on DC Maturation
J.Immunol. 2006 176(3):1386-93.
Timo Ulrichs, Michael Lefmann, Maja Reich, Lars Morawietz, Andreas Roth, Volker Brinkmann, George A Kosmiadi, Peter Seiler, Peter Aichele, Helmut Hahn, Veit Krenn, Ulf B Göbel, Stefan HE Kaufmann
Modified immunohistological staining allows detection of Ziehl-Neelsen-negative Mycobacterium tuberculosis organisms and their precise localization in human tissue.
J.Pathol . 2005. 205(5): 633-40.
Timo Ulrichs, George A Kosmiadi, Vsevolod Trusov, Sabine Jör , Lydia Pradl, Marina Titukhina, Vladimir Mishenko, Nadya Gushina, Stefan HE Kaufmann.
Human tuberculous granulomas induce peripheral lymphoid follicle-like structures to orchestrate local host defence in the lung.
J. Pathol .2004. 204(2):217-28.
Marc Jacobsen, Dirk Repsilber, Andrea Gutschmidt, Albert Neher, Knut Feldmann, Hans J. Mollenkopf, Andreas Ziegler, and Stefan H. E. Kaufmann.
Ras-Associated Small GTPase 33A, a Novel T Cell Factor, Is Down-Regulated in Patients with Tuberculosis.
J.Inf. Dis. 2005. 192(7):1211-8.
Hans-Joachim Mollenkopf , Karin Hahnke , Stefan H.E. Kaufmann.
Transcriptional responses in mouse lungs induced by vaccination with Mycobacterium bovis BCG and infection with Mycobacterium tuberculosis.
Microbes Infect. 8 (2006) 136–144
Stefan H.E. Kaufmann.
Recent findings in immunology give tuberculosis vaccines a new boost. Trends.
Immun ol. 2005. 26(12):660-7.
Helmy Rachman,Michael Strong,Timo Ulrichs,Leander Grode, Johannes Schuchhardt, Hans Mollenkopf,George A. Kosmiadi, David Eisenberg,and Stefan H. E. Kaufmann
Unique Transcriptome Signature of Mycobacterium tuberculosis in Pulmonary Tuberculosis.
Infect. Immun. 2006. 74(2):1233-42
Repsilber D, Fink L, Jacobsen M, Blasing O, Ziegler A.
Sample selection for microarray gene expression studies.
Meth. Inf. Med. 2005;44(3):461-7.
Timo Ulrichs, George A. Kosmiadi, Sabine Jörg, Lydia Pradl, Marina Titukhina, Vladimir Mishenko, Nadya Gushina, and Stefan H. E. Kaufmann.
Differential Organization of the Local Immune Response in Patients with Active Cavitary Tuberculosis or with Nonprogressive Tuberculoma.
J.Inf. Dis. 2005 192(1):89-97.
Martin, C. (2005)
The dream of a vaccine against tuberculosis; new vaccines improving or replacing BCG?
Eur Respir J 26: 162-167.
Brodin P, Majlessi L, Marsollier L, de Jonge MI, Bottai D, Demangel C, Hinds J, Neyrolles O, Butcher PD, Leclerc C, Cole ST, Brosch R.
Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence.
Infect. Immun. 2006, 74(1): 88-98.
Ludovic Tailleux, Nhan Pham-Thi, Anne Bergeron-Lafaurie, Jean-Louis Herrmann, Patricia Charles, Olivier Schwart, Pierre Scheinmann, Philippe H. Lagrange, Jacques de Blic, Abdellatif Tazi, Brigitte Gicquel, Olivier Neyrolles
DC-SIGN Induction in Alveolar Macrophages Defines Privileged Target Host Cells for Mycobacteria in Patients with Tuberculosis.
PLoS Med 2005 2(12):1269-1279
Sylvain Pitarque, Jean-Louis Herrmann, Jean-Luc Duteyrat, Mary Jackson, Graham R. Stewart, François Lecointe, Bruno Payre, Olivier Schwartz, Douglas B. Young, Gilles Marchal, Philippe H. Lagrange, Germain Puzo, Brigitte Giquel, Jérôme Nigou and Olivier Neyrolles.
Deciphering the molecular bases of Mycobacterium tuberculosis binding to the lectin DC-SIGN reveals an underestimated complexity
Biochem. J. 2005. 392 (3) :615-24.
Luis B. Barreiro, Olivier Neyrolles, Chantal L. Babb, Ludovic Tailleux, Hélène Quach, Ken McElreavey, Paul D. van Helden, Eileen G. Hoal, Brigitte Gicquel, Lluis Quintana-Murci.
Promoter Variation in the DC-SIGN–Encoding Gene CD209 Is Associated with Tuberculosis.
PLoS Med 2006, 3(2): 203-235
BrodinP, de JongeMI, MajlessiL, LeclercC, NilgesM, Cole ST and BroschR.
Functional analysis of ESAT-6, the dominant T-cell antigen of Mycobacterium tuberculosis, reveals key residues involved in secretion, complex-formation, virulence and immunogenicity
J. Biol. Chem. 2005, 280(40):33953-59.
Stéphane T. Temmerman, Sammy Place, Anne-Sophie Debrie, Camille Locht, and Françoise Mascart.
Effector functions of heparin-binding hemagglutinin-specific CD8+ T lymphocytes in latent human tuberculosis.
Infect Dis. 2005 Jul 15;192(2):226-32.
Camille Locht, Jean-Michel Hougardy, Carine Rouanet, Sammy Place and Françoise Mascart
Heparin-binding hemagglutinin, from an extrapulmonary dissemination factor to a powerful diagnostic and protective antigen against tuberculosis.
Tuberculosis. 2006. 27 Feb. Epub ahead of print.
Leander Grode, Peter Seiler, Sven Baumann, Jürgen Hess, Volker Brinkmann, Ali Nasser Eddine, Peggy Mann, Christian Goosmann, Silke Bandermann, Debbie Smith, Gregory J. Bancroft, Jean-Marc Reyrat, Dick van Soolingen, Bärbel Raupach and Stefan H.E. Kaufmann.
Increased vaccine efficacy against tuberculosis of recombinant Mycobacterium bovis bacille Calmette-Guérin mutants that secrete listeriolysin.
J.Clin. Invest. 2005. 115:2472-2479
Nadia Caccamo, Serena Meraviglia, Viviana Ferlazzo, Daniela Angelini, Giovanna Borsellino, Fabrizio Poccia, Luca Battistini, Francesco Dieli, Alfredo Salerno
Differential requirements for antigen or homeostatic cytokines for proliferation and differentiation of human Vγ9δ2 naive, memory and effector T cell subsets.
Eur. J.Immunol. 2005 35(6):1764-72.
Gonzalo Asensio J, Maia C, Ferrer NL, Barilone N, Laval F, Soto CY, Winter N, Daffe M, Gicquel B, Martin C, Jackson M.
The virulence-associated two-component PhoP-PhoR system controls the biosynthesis of polyketide-derived lipids in Mycobacterium tuberculosis.
J. Biol. Chem. Epub 2005 Dec 2 (2006. 281 (3):1313-6.
Arun T. Kamath, Uli Fruth, Michael J. Brennan, Roland Dobbelaer, Peter Hubrechts, Mei Mei Ho, Ronald E. Mayner, Jelle Thole, K. Barry Walker, Margaret Liu and Paul-Henri Lambert
New live mycobacterial vaccines: the Geneva consensus on essential steps towards clinical development.
Vaccine 2005: 23(29):3753-61
Henri de la Salle,Sabrina Mariotti,Catherine Angenieux,Martine Gilleron,Luis-Fernando Garcia-Alles, Dag Malm,Thomas Berg, Samantha Paoletti,Blandine Maître,Lionel Mourey,Jean Salamero,Jean Pierre Cazenave, Daniel Hanau,Lucia Mori,Germain Puzo,Gennaro De Libero
Assistance of Microbial Glycolipid Antigen Processing by CD1e.
Science 2005 :310. (5752): 1321 – 1324.
Infect. Immun. 77: 622-631 (2009)