Publications 2017

The Cross-Species Mycobacterial Growth Inhibition Assay (MGIA) Project, 2010-2014.
Brennan MJ, Tanner R, Morris S, Scriba TJ, Achkar JM, Zelmer A, Hokey DA, Izzo A, Sharpe S, Williams A, Penn-Nicholson A, Erasmus M, Stylianou E, Hoft DF, McShane H, Fletcher HA
Clin Vaccine Immunol. 2017 Sep 5;24(9). pii: e00142-17. doi: 10.1128/CVI.00142-17

Optimatisation of a murine splenocyte mycobacterial growth inhibition assay using virulent Mycobacterium tuberculosis
Christina Jensen, Line Lindebo Holm, Erik Svensson, Claus Aagaard & Morten Ruhwald
Nature Communications volume8, Article number: 16085 (2017)  doi:10.1038/ncomms16085

Reactogenicity to major tuberculosis antigens absent in BCG is linked to improved protection against Mycobacterium tuberculosis

Nacho Aguilo, Jesus Gonzalo-Asensio, Samuel Alvarez-Arguedas, Dessislava Marinova, Ana Belen Gomez, Santiago Uranga, Ralf Spallek, Mahavir Singh, Regine Audran, Francois Spertini & Carlos Martin

Variable BCG efficacy in rhesus populations: Pulmonary BCG provides protection where standard intra-dermal vaccination fails

Frank A.W. Verreck, Elma Z. Tchilian, Richard A.W. Vervenne, Claudia C. Sombroek, Ivanela Kondova, Okke A. Eissen, Vinod Sommandas, Nicole M. van der Werff, Ernst Verschoor, Gerco Braskamp, Jaco Bakker, Jan A.M. Langermans, Peter J. Heidt, Tom H.M. Ottenhoff, Klaas W. van Kralingen, Alan W. Thomas, Peter C.L. Beverley, Clemens H.M. Kocken

Discovery of the type VII ESX-1 secretion needle?
Ates LS, Brosch R.
Mol Microbiol. 2017 Jan;103(1):7-12.

Mycobacterium tuberculosis Complex Exhibits Lineage-Specific Variations Affecting Protein Ductility and Epitope Recognition.
Yruela I, Contreras-Moreira B, Magalhães C, Osório NS, Gonzalo-Asensio J.
Genome Biol Evol. 2017 Jan 6.

Human and Mouse Hematopoietic Stem Cells Are a Depot for Dormant Mycobacterium tuberculosis.
Tornack J, Reece ST, Bauer WM, Vogelzang A, Bandermann S, Zedler U, Stingl G, Kaufmann SH, Melchers F.
PLoS One. 2017 Jan 3;12(1):e0169119. doi: 10.1371/journal.pone.0169119. eCollection 2017.

Publications 2016

Lack of Response to HBHA in HIV-Infected Patients with Latent Tuberculosis Infection.
Delogu G, Vanini V, Cuzzi G, Chiacchio T, De Maio F, Battah B, Pinnetti C, Sampaolesi A, Antinori A, Goletti D.
Scand J Immunol. 2016 Dec;84(6):344-352. doi: 10.1111/sji.12493.

C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells
Anthony Troegelera, Ingrid Merciera, Céline Cougoulea, Danilo Pietrettia, André Coloma, Carine Duvala, Thien-Phong Vu Manhc, Florence Capillad, Renaud Poinclouxa, Karine Pingrisa, Jérôme Nigoua, Jörg Rademanne, Marc Dalodc, Frank A. W. Verreckf, Talal Al Saatid, Geanncarlo Lugo-Villarinoa, Bernd Lepeniesg, Denis Hudrisiera and Olivier Neyrollesa
PNAS Dec 2016, doi: 10.1073/pnas.1613254114

Correlates of tuberculosis risk: predictive biomarkers for progression to active tuberculosis.
Petruccioli E, Scriba TJ, Petrone L, Hatherill M, Cirillo DM, Joosten SA, Ottenhoff TH, Denkinger CM, Goletti D.
Eur Respir J. 2016 Dec;48(6):1751-1763. 

High-throughput and computational approaches for diagnostic and prognostic host tuberculosis biomarkers.
Weiner J, Kaufmann SHE
Journal of Infectious Diseases (2016), DOI:

Novel approaches to TB vaccine development.
Stefan H.E. Kaufmanna, ,January Weinera, C. Fordham von Reynb
International Journal of Infectious Diseases Nov 2016. DOI:

Lipase Processing of Complex Lipid Antigens.
Sander P, Becker K, Molin MD.
Cell Chem Biol. 2016 Sep 22;23(9):1044-6.

Inflammatory Properties and Adjuvant Potential of Synthetic Glycolipids Homologous to Mycolate Esters of the Cell Wall of Mycobacterium tuberculosis.
Tima HG, Al Dulayymi JR, Denis O, Lehebel P, Baols KS, Mohammed MO, L’Homme L, Sahb MM, Potemberg G, Legrand S, Lang R, Beyaert R, Piette J, Baird MS, Huygen K, Romano M.
J Innate Immun. 2016 Nov 18.

Microfluidics based manufacture of liposomes simultaneously entrapping hydrophilic and lipophilic drugs.
Joshi, Sameer and Hussain, Maryam T and Roces, Carla B and Anderluzzi, Giulia and Kastner, Elisabeth and Salmaso, Stefano and Kirby, Daniel J and Perrie, Yvonne.
International Journal of Pharmaceutics, 2016, ISSN 0378-5173

Rapid quantification and validation of lipid concentrations within liposomes.
Carla B Roces, Elisabeth Kastner, Peter Stone , Deborah Lowry and Yvonne Perrie.
Pharmaceutics 2016, 8(3), 29

Type VII Secretion Systems in Gram-Positive Bacteria.
Daria Bottai, Matthias I. Gröschel, Roland Brosch
“Protein Export in Gram-positive Bacteria”, within the book series  “Current Topics in Microbiology and Immunology” (CTMI), Springer;
Chapter, Part of the series Current Topics in Microbiology and Immunology pp 1-31, 2016

Sleeping Beauty and the Story of the Bacille Calmette-Guérin Vaccine.
Fletcher HA.
MBio. 2016 Aug 30;7(4). pii: e01370-16.

Mycobacterium tuberculosis components expressed during chronic infection of the lung contribute to long-term control of pulmonary tuberculosis in mice.
Claudio Counoupas, Rachel Pinto, Gayathri Nagalingam, Grant A Hill-Cawthorne, Carl G Feng, Warwick J Britton & James A Triccas.
npj Vaccines 1, Article number: 16012 (2016)

Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria.
Boritsch EC, Khanna V, Pawlik A, Honoré N, Navas VH, Ma L, Bouchier C, Seemann T, Supply P, Stinear TP, Brosch R.
Proc Natl Acad Sci U S A. 2016 Aug 30;113(35):9876-81.

ESX secretion systems: mycobacterial evolution to counter host immunity.
Gröschel MI, Sayes F, Simeone R, Majlessi L, Brosch R.
Nat Rev Microbiol. 2016 Nov;14(11):677-691.

Mycobacterium tuberculosis lipoproteins in virulence and immunity – fighting with a double-edged sword.
Becker K, Sander P.
FEBS Lett, 590: 3800–3819.

TB biomarkers, TB correlates and human challenge models: New tools for improving assessment of new TB vaccines.
Kaufmann SH, Fortune S, Pepponi I, Ruhwald M, Schrager LK, Ottenhoff TH.
Tuberculosis (Edinb). 2016 Aug;99 Suppl 1:S8-S11

CD4+ T Cells Recognizing PE/PPE Antigens Directly or via Cross Reactivity Are Protective against Pulmonary Mycobacterium tuberculosis Infection.
Sayes F, Pawlik A, Frigui W, Gröschel MI, Crommelynck S, Fayolle C, Cia F, Bancroft GJ, Bottai D, Leclerc C, Brosch R, Majlessi L.
PLoS Pathog. 2016 Jul 28;12(7):e1005770.

Innate signaling by mycobacterial cell wall components and relevance for development of adjuvants for subunit vaccines.
Tima HG, Huygen K, Romano M.
Expert Rev Vaccines. 2016 Nov;15(11):1409-1420.

Use of several immunological markers to model the probability of active tuberculosis.
Petruccioli E, Navarra A, Petrone L, Vanini V, Cuzzi G, Gualano G, Palmieri F, Girardi E, Goletti D.
Diagn Microbiol Infect Dis. 2016 Oct;86(2):169-71

A Mouse Model of Latent Tuberculosis Infection to Study Intervention Strategies to Prevent Reactivation.
Andreas Kupz , Ulrike Zedler, Manuela Stäber, Stefan H. E. Kaufmann.
PLoS One. 2016; 11(7): e0158849.

Syndecans promote mycobacterial internalization by lung epithelial cells.
Zimmermann N, Saiga H, Houthuys E, Moura-Alves P, Koehler A, Bandermann S, Dorhoi A, Kaufmann SH.
Cell Microbiol. 2016 Jun 9.

Tuberculosis Biomarkers: From Diagnosis to Protection.
Delia Goletti, Elisa Petruccioli, Simone A. Joosten, and Tom H.M. Ottenhoff.
Infect Dis Rep. 2016 Jun 24; 8(2): 6568.

Patients with Tuberculosis Have a Dysfunctional Circulating B-Cell Compartment, Which Normalizes following Successful Treatment.
Joosten SA, van Meijgaarden KE, Del Nonno F, Baiocchini A, Petrone L, Vanini V, Smits HH, Palmieri F, Goletti D, Ottenhoff TH.
PLoS Pathog. 2016 Jun 15;12(6):e1005687.

Granzyme A Is Expressed in Mouse Lungs during Mycobacterium tuberculosis Infection but Does Not Contribute to Protection In Vivo.
Uranga S, Marinova D, Martin C, Pardo J, Aguilo N.
PLoS One. 2016 Apr 7;11(4):e0153028.

Deletion of nuoG from the Vaccine Candidate Mycobacterium bovis BCG ΔureC::hly Improves Protection against Tuberculosis.
Gengenbacher 1, Nieuwenhuizen N, Vogelzang A, Liu H, Kaiser P, Schuerer S, Lazar D, Wagner I, Mollenkopf HJ, Kaufmann SH.
MBio. 2016 May 24;7(3). pii: e00679-16

Perspectives on mycobacterial vacuole-to-cytosol translocation: the importance of cytosolic access.
Simeone R, Majlessi L, Enninga J, Brosch R.
Cell Microbiol. 2016 Aug;18(8):1070-7.

Vaccination against tuberculosis with whole cell 1 mycobacterial vaccines.
Thomas J. Scriba, Stefan H.E. Kaufmann, Paul Henri Lambert, Melvin Sanicas, Carlos Martin, Olivier Neyrolles.
J Infect Dis. 2016 Sep 1;214(5):659-64.

Innate signaling by mycobacterial cell wall components and relevance for development of adjuvants for subunit vaccines.
Tima HG, Huygen K, Romano M.
Expert Rev Vaccines. 2016 May 30:1-12. [Epub ahead of print]

Post-exposure vaccination with the vaccine candidate Bacillus Calmette–Guérin ΔureC::hly induces superior protection in a mouse model of subclinical tuberculosis
Martin Gengenbacher Peggy Kaiser, Stefanie Schuerer, Doris Lazar, Stefan H.E. Kaufmann
Microbes Infect 2016 May 17;18(5):364-8. Epub 2016 Mar 17. doi:10.1016/j.micinf.2016.03.005

Vaccination in early life: standing up to the challenges
Elodie Mohr, Claire-Anne Siegrist
Opinion in Immunology, Volume 41, August 2016, Pages 1–8 doi:10.1016/j.coi.2016.04.004

Granzyme A Is Expressed in Mouse Lungs during Mycobacterium tuberculosis Infection but Does Not Contribute to Protection In Vivo
Santiago Uranga, Dessislava Marinova, Carlos Martin, Julián Pardo, Nacho Aguilo
PLOS, April 7, 2016

BCG Vaccination Induces Robust CD4+ T Cell Responses to Mycobacterium tuberculosis Complex-Specific Lipopeptides in Guinea Pigs.
Kaufmann E, Spohr C, Battenfeld S, De Paepe D, Holzhauser T, Balks E, Homolka S, Reiling N, Gilleron M, Bastian M.
J Immunol. 2016 Mar 15;196(6):2723-32. doi: 10.4049/jimmunol.1502307. Epub 2016 Feb 17.

The BCG Strain Pool: Diversity Matters.
Bottai D, Brosch R.
Mol Ther. 2016 Feb;24(2):201-3. doi: 10.1038/mt.2016.18.

Lipoarabinomannan-responsive Polycytotoxic T Cells are Associated With Protection in Human Tuberculosis.
Busch M, Herzmann C, Kallert S, Zimmermann A, Höfer C, Mayer D, Zenk SF, Muche R, Lange C, Bloom BR, Modlin RL, Stenger S; TBornotTB Network.
Am J Respir Crit Care Med. 2016 Feb 16. [Epub ahead of print]

Towards new TB vaccines: what are the challenges?
Dockrell HM.
Pathog Dis. 2016 Mar 9. pii: ftw016. [Epub ahead of print]

Molecular Determinants in Phagocyte-Bacteria Interactions.
Kaufmann SH, Dorhoi A.
Immunity. 2016 Mar 15;44(3):476-91. doi: 10.1016/j.immuni.2016.02.014.

Pathology and immune reactivity: understanding multidimensionality in pulmonary tuberculosis.
Dorhoi A, Kaufmann SH.
Semin Immunopathol. 2016 Mar;38(2):153-66. doi: 10.1007/s00281-015-0531-3. Epub 2015 Oct 5.

On the impact of masking and blocking hypotheses for measuring the efficacy of new tuberculosis vaccines.
Arregui S, Sanz J, Marinova D, Martín C, Moreno Y.
PeerJ. 2016 Feb 11;4:e1513. doi: 10.7717/peerj.1513. eCollection 2016.

Reprogramming the T Cell Response to Tuberculosis
Joshua S. Woodworth, Peter Andersen
Cell, DOI:

Mycobacterial pan-genome analysis suggests important role of plasmids in the radiation of type VII secretion systems.
Dumas E, Boritsch EC, Vandenbogaert M, Rodríguez de la Vega RC, Thiberge JM, Caro V, Gaillard JL, Heym B, Girard-Misguich F, Brosch R, Sapriel G.
Genome Biol Evol. 2016 Jan 8. pii: evw001.

Inflammation and the coagulation system in tuberculosis: Tissue Factor leads the dance.
Caccamo N, Dieli F.
Eur J Immunol. 2016 Jan 14. doi: 10.1002/eji.201546225.

Publications 2015

Pathology and immune reactivity: understanding multidimensionality in pulmonary tuberculosis.
Dorhoi A, Kaufmann SH.
Semin Immunopathol. 2015 Oct 5.

“Protein Export in Gram-positive Bacteria”, within the book series “Current Topics in Microbiology and Immunology” (CTMI), Springer;
Volume editors: Fabio Bagnoli and Rino Rappuoli
Chapter: Type VII secretion systems in Gram-positive bacteria
Daria Bottai, Matthias I. Gröschel, and Roland Brosch

Detection of IgG1 antibodies against Mycobacterium tuberculosis DosR and Rpf antigens in tuberculosis patients before and after chemotherapy
Ana Márcia Menezes Mattos, Alexandre Silva Chaves, Kees L.M.C. Franke, Bárbara Bruna Muniz Figueiredo, Ana Paula Ferreira, Tom H.M. Ottenhoff, Henrique Couto Teixeira
Tuberculosis , Volume 96 , 65 – 70, DOI:

Analysis of MHC immunopeptidomes using mass spectrometry.
Caron E, Kowalewski DJ, Koh CC, Sturm T, Schuster H, Aebersold R.
Mol Cell Proteomics. 2015 Oct 19. pii: mcp.O115.052431. [Epub ahead of print]

Versatile myeloid cell subsets contribute to tuberculosis-associated inflammation.
Dorhoi A, Kaufmann SH.
Eur J Immunol. 2015 Aug;45(8):2191-202.

An open-source computational and data resource to analyze digital maps of immunopeptidomes.
Caron E, Espona L, Kowalewski DJ, Schuster H, Ternette N, Alpízar A, Schittenhelm RB, Ramarathinam SH, Lindestam Arlehamn CS, Chiek Koh C, Gillet LC, Rabsteyn A, Navarro P, Kim S, Lam H, Sturm T, Marcilla M, Sette A, Campbell DS, Deutsch EW, Moritz RL, Purcell AW, Rammensee HG, Stevanovic S, Aebersold R.
Elife. 2015 Jul 8;4. doi: 10.7554/eLife.07661.

Evolutionary Landscape of the Mycobacterium tuberculosis Complex from the Viewpoint of PhoPR: Implications for Virulence Regulation and Application to Vaccine Development
Esther Broseta, Carlos Martín, Jesús Gonzalo-Asensio
doi: 10.1128/mBio.01289-15 20 October 2015 mBio vol. 6 no. 5 e01289-15

MTBVAC vaccine is safe, immunogenic and confers protective efficacy against Mycobacterium tuberculosis in newborn mice
Nacho Aguilo, Santiago Uranga, Dessislava Marinova, Marta Monzon, Juan Badiola, Carlos Martin

Pulmonary but Not Subcutaneous Delivery of BCG Vaccine Confers Protection to Tuberculosis-Susceptible Mice by an Interleukin 17-Dependent Mechanism.
Aguilo N, Alvarez-Arguedas S, Uranga S, Marinova D, Monzón M, Badiola J, Martin C.
J Infect Dis. 2015 Oct 22. pii: jiv503. [Epub ahead of print]

Immunological Signatures Identifying Different Stages of Latent Mycobacterium tuberculosis Infection and Discriminating Latent from Active Tuberculosis in Humans.
Smits K, Corbière V, Dirix V, Mekkaoui L, Wyndham-Thomas C, et al. (2015)
J Clin Cell Immunol 6:341. doi:10.4172/2155-9899.1000341

Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers
Maria M. Esterhuyse, January Weiner 3rd, Etienne Caron, Andre G. Loxton, Marco Iannaccone, Chandre Wagman, Philippe Saikali, Kim Stanley, Witold E. Wolski, Hans-Joachim Mollenkopf, Matthias Schick, Ruedi Aebersold, Heinz Linhart, Gerhard Walzl, Stefan H. E. Kaufmann
doi: 10.1128/mBio.01187-15, 15 September 2015 mBio vol. 6 no. 5 e01187-15

Synthetic Long Peptide Derived from Mycobacterium tuberculosis Latency Antigen Rv1733c Protects against Tuberculosis.
Coppola M, van den Eeden SJ, Wilson L, Franken KL, Ottenhoff TH, Geluk A.
Clin Vaccine Immunol. 2015 Sep;22(9):1060-9. doi: 10.1128/CVI.00271-15. Epub 2015 Jul 22.

Molecular signatures for vaccine development.
Maertzdorf J, Kaufmann SH, Weiner J 3rd.
Vaccine. 2015 Apr 6. pii: S0264-410X(15)00394-1. doi: 10.1016/j.vaccine.2015.03.075. [Epub ahead of print]

Protein energy malnutrition during vaccination has limited influence on vaccine efficacy but abolishes immunity if administered during Mycobacterium tuberculosis infection.
Hoang T, Agger EM, Cassidy JP, Christensen JP, Andersen P.
Infect Immun. 2015 Mar 9. pii: IAI.03030-14. [Epub ahead of print]

Liposomal delivery of lipoarabinomannan triggers Mycobacterium tuberculosis specific T-cells.
Kallert s, Zenk SF, Walther P, Grieshober M, Weil T, Stenger S.
Tuberculosis (Edinb). 2015 Jul;95(4):452-62. doi: 10.1016/ Epub 2015 May 19.

Molecular signatures for vaccine development.
Maertzdorf J, Kaufmann SH, Weiner J 3rd.
Vaccine. 2015 Apr 6. pii: S0264-410X(15)00394-1. doi: 10.1016/j.vaccine.2015.03.075. [Epub ahead of print]

High-throughput data analysis and data integration for vaccine trials.
Weiner J 3rd, Kaufmann SH, Maertzdorf J.
Vaccine. 2015 May 11. pii: S0264-410X(15)00593-9. doi: 10.1016/j.vaccine.2015.04.096. [Epub ahead of print]